[1]钟文英,梁宏唏,丁敏,等.胰高血糖素免疫敏感膜的制备及SPR检测[J].东南大学学报(自然科学版),1999,29(6):48-51.[doi:10.3969/j.issn.1001-0505.1999.06.011]
 Zhong Wenying,Liang Hongxi,Ding Min,et al.Preparation of Glucagon Immunosensitive Film for SPR Analysis[J].Journal of Southeast University (Natural Science Edition),1999,29(6):48-51.[doi:10.3969/j.issn.1001-0505.1999.06.011]
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胰高血糖素免疫敏感膜的制备及SPR检测()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
29
期数:
1999年第6期
页码:
48-51
栏目:
生物医学工程
出版日期:
2000-11-20

文章信息/Info

Title:
Preparation of Glucagon Immunosensitive Film for SPR Analysis
作者:
钟文英1 梁宏唏1 丁敏1 顾建华2 陆祖宏2
1 中国药科大学分析化学教研室,南京 210009; 2 东南大学分子电子学实验室,南京 210096
Author(s):
Zhong Wenying1 Liang Hongxi1 Ding Min1 Gu Jianhua2 Lu Zuhong2
1 Division of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009
2 National Laboratory of Molecular and Biomolecular Electronics, Southease University, Nanjing 210096
关键词:
胰高血糖素 免疫敏感膜 表面等离激元共振
Keywords:
glucagon immunosensitive film surface plasmon resonance (SPR)
分类号:
Q578
DOI:
10.3969/j.issn.1001-0505.1999.06.011
摘要:
制备胰高血糖素免疫敏感膜并建立一种快速、简便测定胰高血糖素含量的方法.利用化学自组装技术将胰高血糖素的抗体组装到SPR基片上,首先通过对已知浓度的胰高血糖素进行SPR检测,确定其结合常数,并以此对未知浓度的胰高血糖素进行了测定,样品无需纯化和标记.
Abstract:
A glucagon immunosensitive film was prepared for the rapid determination of glucagon concentration with a simple and convenient method based on SPR technique.With self-assembly technique,antibody against glucagon is immobilized on SPR substrates and reacted with glucagon in solutions. By calculating the binding kinetic parameters of the glucagon solution of unknown concentration, the concentration could be determined. With this method, the component of sample can be analyzed without special purification and labeling.

参考文献/References:

[1] 裴的善.胰高血糖素的临床应用.中国医药学杂志,1989,24(4):204
[2] 杨利国,胡少昶,魏平华,等.酶联免疫测定技术.南京:南京大学出版社,1998.120
[3] Stenbeg E,Persson B,Roos H,et al.Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins.Journal of Colloid and Interface Science,1991,143(2):513
[4] Karlsson R,Michaelsson A,Mattsson L.Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system.J Immunol Method,1991,145(1):229
[5] Stephen D,Kathy M,Johnsson V,et al.Studies on the interaction of staphylococcus aureus and staphylococcus epidermidis with fibronectin using surface plasmon resonance(BIAcore).Journal of Microbiological Methods,1997,28(1):77

备注/Memo

备注/Memo:
第一作者:女,1968年生,硕士,讲师.
更新日期/Last Update: 1999-11-20