[1]唐祖明,郑纪山,肖中党,等.甲肝病毒单克隆抗体的研制与应用[J].东南大学学报(自然科学版),2000,30(5):21-24.[doi:10.3969/j.issn.1001-0505.2000.05.005]
 Tang Zuming,Zheng Jishan,Xiao Zhongdang,et al.Preparation and Application of Monoclonal Antibody to Hepatitis A Virus[J].Journal of Southeast University (Natural Science Edition),2000,30(5):21-24.[doi:10.3969/j.issn.1001-0505.2000.05.005]
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甲肝病毒单克隆抗体的研制与应用()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
30
期数:
2000年第5期
页码:
21-24
栏目:
生物医学工程
出版日期:
2000-09-20

文章信息/Info

Title:
Preparation and Application of Monoclonal Antibody to Hepatitis A Virus
作者:
唐祖明1 郑纪山2 肖中党1 周宗安2 陆祖宏1
1 东南大学分子与生物分子电子学实验室,南京 210096; 2 南京军区军事医学研究所,南京 210002
Author(s):
Tang Zuming1 Zheng Jishan2 Xiao Zhongdang1 Zhou Zongan2 Lu Zuhong1
1 National Laboratory of Molecular and Biomolecular Electronics, Southeast University, Nanjing 210096
2 Institute of Military Medicine, Nanjing Command, Nanjing 210002
关键词:
甲肝病毒 单克隆抗体 细胞融合 免疫荧光法
Keywords:
hepatitis A virus monoclonal antibody hybridoma immunofluorescence assay
分类号:
R392-33
DOI:
10.3969/j.issn.1001-0505.2000.05.005
摘要:
本研究成功制备了抗甲肝病毒(HAV) McAb,为甲肝研究和诊断提供了大量高效价标准化抗体.用细胞培养的NJ-3株HAV免疫Balb/c小鼠,经细胞融合技术、IFA筛选和有限稀释克隆,建立了5株能稳定分泌抗HAV McAb的杂交瘤细胞.经鉴定,其上清和腹水的抗体效价分别为0.5×102~1×103及1×103~2×105.用免疫印迹法和间接ELISA分析,这5株抗体分别能与HAV的3个抗原位点结合.在HAAg检测中,M
Abstract:
To obtaining Monoclonal Antibody(McAbs) against hepatitis A virus(HAV), Balb/c mice were immunized with HAV NJ-3 strain from cell culture, then lymphocytes from the splenic tissues of the mice were fused with Sp2/0 myeloma cell. Five lines of hybridoma cell secreting McAbs to HAV were established by screening with IFA, limiting dilution technique and expanded into larger cell populations. The titer of McAbs in supernatant of culture and ascites fluids were 0.5×102-1×103 and 1×103-2×105,respectively. These McAbs could recognize three different epitopes of HAV-antigen. Using the McAbs to test for anti-HAV IgM and HAAg could simplify procedure.

参考文献/References:

[1] Bishop N E,Hugo D L,Borovec S V,et al.Rapid and efficient purification of hepatitis A virus from cell culture.J Virol Methods,1994,47(3):203~216
[2] 郑纪山,林建汶,李法卿,等.从人外周血单个核白细胞分离甲型肝炎病毒.病毒学杂志,1988(3):285~289
[3] Siegl G,Frosner G G.Characterization and classification of virus particles associated with hepatitis A.I.size,density and sedimentation.J Virol,1978,26(1):40~47
[4] Hughes J V,Stanton L W,Tomassini J E,et al.Neutralizing monoclonal antibodies to hepatitis A virus:partial localization of a neutralizing antigenic site.J Virol,1984,52(2):465~473

备注/Memo

备注/Memo:
第一作者:男, 1950年生, 硕士, 副教授.
更新日期/Last Update: 2000-09-20