[1]肖鹏峰,何农跃,朱纪军,等.分子印章法DNA芯片的合成(Ⅰ)——PDMS印章的固定与收缩研究[J].东南大学学报(自然科学版),2001,31(2):6-10.[doi:10.3969/j.issn.1001-0505.2001.02.002]
 Xiao Pengfeng,He Nongyue,Zhu Jijun,et al.DNA Microarray Synthesis Using PDMS Molecular Stamps (Ⅰ): The Fixation and Shrinkage of PDMS Molecular Stamps[J].Journal of Southeast University (Natural Science Edition),2001,31(2):6-10.[doi:10.3969/j.issn.1001-0505.2001.02.002]
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分子印章法DNA芯片的合成(Ⅰ)——PDMS印章的固定与收缩研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
31
期数:
2001年第2期
页码:
6-10
栏目:
化学化工
出版日期:
2001-03-20

文章信息/Info

Title:
DNA Microarray Synthesis Using PDMS Molecular Stamps (Ⅰ): The Fixation and Shrinkage of PDMS Molecular Stamps
作者:
肖鹏峰1 何农跃1 朱纪军1 徐吉庆2 陆祖宏1
1 东南大学分子与生物分子电子学教育部重点实验室, 南京 210096; 2 南京益来基因医学有限公司, 南京 210016
Author(s):
Xiao Pengfeng1 He Nongyue1 Zhu Jijun1 Xu Jiqing2 Lu Zuhong1
1 Key Laboratory for Molecular and Bio-Molecular Electronics of Ministry of Education, Southeast University, Nanjing 210096; 2 Nanjing E-life Gene Medicine Co. Ltd., Nanjing 210016)
关键词:
PDMS印章 固定 收缩 DNA芯片
Keywords:
PDMS stamp fixation shrinkage DNA microarray
分类号:
O634.4
DOI:
10.3969/j.issn.1001-0505.2001.02.002
摘要:
用Micron XYZ Scope考察了用不同方法处理的玻璃片对膜厚为0.6~1.0 mm, 阵点高度为15 μm的聚二甲基硅氧烷(PDMS)印章的粘附固定情况以及对印章收缩的影响.经硅烷化的玻璃片与PDMS印章结合牢固,虽然印章表面阵点的面积收缩为0.286%,但由于分子间的强相互作用力以及分子之间的铆合作用,使其印章的线收缩为0.040 3%.这一结论保证了寡核苷酸合成过程中一套印章在合成载片位点上的多次准确定位以及印章多次压印偶联反应不发生错位,亦即为分子印章法DNA芯片的正确合成提供了依据.
Abstract:
Because the shrinkage of poly(dimethylsiloxane) (PDMS) stamps may give rise to variation in stamping, the fixation of the PDMS stamps to glass slides is of significant importance in accurate overlapping of patterns of different stamps in the in situ synthesis of oligonucleotide microarrays on glass slides by means of molecule stamp method. In this paper, the effect of different methods to treat glass slide on the fixation of PDMS stamps was investigated. Detected by Micron XYZ Scope (Nissho Seimisu Kogaku Co. Ltd.), the PDMS films investigated possess a thickness of 0.6-1.0 mm and the height of its microarray dot was 15 μm. It was found that the fixation of the PDMS stamps formed on the silanized glass slides stuck to the glass slides more strongly than those by other methods. Although the shrinkage of a single dot of PDMS stamp microarray could be controlled within 0.286% in area, the shrinkage of the whole PDMS stamp film was 0.040 3% in linearity due to its very strong stick to the glass slides. This small change in PDMS stamps area could guarantee the accurate overlap of the patterns of a set stamps with which the DNA microarrays was synthesized in situ on glass slides.

参考文献/References:

[1] Xia Younan,Whitesides G M.Soft lithography.Angew Chem Int Ed,1998,37:550~575
[2] Chauhury M K,Whitesides G M.Correlation between surface free energy and surface constitution.Science,1992,255:1230~1232
[3] Marzolin C,Terfort A,Whitesides G M.Patterning of a polysiloxane precursor to silicate glasses by microcontact printing.Thin Solid Films,1998,315:9~12
[4] Tao Deng,Goetting L B,Hu Junmin,et al.Microfabrication of half-wave rectifier circuits using soft lithography.Sensors and Actuators A:Physical,1999,75:60~64
[5] Kumar A,Whitesides G M.Features of gold having micrometer to centimeter dimensions can be formed through a combination of stamping with an elastometric stamp and an alkanethiol “ink” followed by chemical etching.Appl Phys Lett,1993,63(14):2002~2004
[6] Xia Younan,Kim E,Zhao Xiaomei.Complex optical surfaces formed by replica molding against elastomeric masters.Science,1996,237:347~349
[7] Zhao Xiaomei,Xia Younan,Whitesides G M.Fabrication of three-dimensional micro-structures:microtransfer molding.Advanced Materials,1996,8:837~840
[8] Kim Enoch,Xia Younan,Whitesides G M.Polymer microstructures formed by molding.Nature,1995,376:581~584
[9] Kim Enoch,Xia Younan,Zhao Xiaomei,et al.Solvent-assisted microcontact moldings:a convenient method for fabricating three-dimensional structures on surfaces of polymers.Advanced Materials,1997,9:581~584
[10] Ferguson G S,Chauhury M K,Sigal G B,et al.Contact adhesion of thin gold films on elastomeric supports:cold welding under ambient conditions.Science,1991,253:776~778
[11] 陆祖宏.制备化合物微阵列芯片的方法及其由该方法制备的化合物微阵列芯片.中国专利,CN98 111220X.1999-01-27
[12] Martin B D,Gaber B P,Charles H,et al.Direct protein fabrication using a hydrogel “stamper”.Langmuir,1998,14(15):3971~3975
[13] M St John Pamela,Craighead H G.Microcontact printing and pattern transfer using trichorosilances on oxide substrates.Appl Phys Lett,1996,68(7):1022~1024
[14] 陆祖宏.生物芯片的研究、发展和应用.电子科技导报,1998,11:1~5

备注/Memo

备注/Memo:
作者简介:肖鹏峰,男,1963年生,副教授,博士研究生.
基金项目:国家863计划(103-13-05-01,101-01-01)、973计划(G1998051204)、国家自然科学基金(60071001)和教育部“高等学校骨干教师资助计划”资助项目.
更新日期/Last Update: 2001-03-20