[1]王轶文,徐吉庆,陈扬,等.电解致酸脱保护DNA在片合成研究[J].东南大学学报(自然科学版),2002,32(1):77-80.[doi:10.3969/j.issn.1001-0505.2002.01.018]
 Wang Yiwen,Xu Jiqing,Chen Yang,et al.In situ synthesis of DNA microarray by means of deprotection with acid by electrolysis[J].Journal of Southeast University (Natural Science Edition),2002,32(1):77-80.[doi:10.3969/j.issn.1001-0505.2002.01.018]
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电解致酸脱保护DNA在片合成研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
32
期数:
2002年第1期
页码:
77-80
栏目:
化学化工
出版日期:
2002-01-20

文章信息/Info

Title:
In situ synthesis of DNA microarray by means of deprotection with acid by electrolysis
作者:
王轶文1 徐吉庆2 陈扬1 何农跃1
1 东南大学分子与生物分子电子学教育部重点实验室,南京 210096; 2 南京益来基因医学有限公司, 南京 210016
Author(s):
Wang Yiwen1 Xu Jiqing2 Chen Yang1 He Nongyue1
1 Key Laboratory for Molecular and Bio-molecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China
2 Nanjing E-life Gene Medicine Co Ltd., Nanjing 210016, China
关键词:
基因芯片 在片合成 电解致酸脱保护 金片硫醇自组装 DMT紫外吸收
Keywords:
genechip in situ synthesis deprotection with acid by electrolysis thiol self-assembled DMT UV absorption
分类号:
O643.4
DOI:
10.3969/j.issn.1001-0505.2002.01.018
摘要:
提出了以电解致酸脱保护法为特征的电助DNA在片合成新方法.在2.0 V电解电位下电解水,释放氢离子并富集于阳极极板附近,导致局部酸度增强,使核酸单体分子发生脱保护反应.测定了该合成方法的合成产率与电解时间,支持电解质浓度,水浓度等条件的关系.结果表明,电解时间越长,脱保护效率越高,两者基本呈线性关系; 支持电解质浓度与水浓度等条件对脱保护效率基本无影响.
Abstract:
In order to develop a new approach to prepare high-density genechip with in situ synthesis, the authors propose a way to synthesize DNA microarrays by means of deprotection with acid generated by electrolyzing some substances in solvent. Water can release protons when electrolyzed at 2.0 V, leading to the increase of acidity on anode. Therefore, DMT protecting group can be removed. The relationship between the synthesis efficiency and other conditions, such as electrolysis time, concentration of electrolyte, concentration of water and so on was measured. It was found that the longer the electrolysis time, the higher the deprotection efficiency. The concentrations of electrolyte and water have little influence on deprotection efficiency.

参考文献/References:

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相似文献/References:

[1]孙啸,王晔,张晓莉,等.基因芯片设计及数据分析软件系统[J].东南大学学报(自然科学版),2000,30(5):1.[doi:10.3969/j.issn.1001-0505.2000.05.001]
 Sun Xiao,Wang Ye,Zhang Xiaoli,et al.A Software System of Gene Chip Design and Data Analysis[J].Journal of Southeast University (Natural Science Edition),2000,30(1):1.[doi:10.3969/j.issn.1001-0505.2000.05.001]

备注/Memo

备注/Memo:
基金项目: 国家863高技术项目(103-13-05-01,101-01-01)、国家重大基础研究项目(G1998051204)、国家自然科学基金资助项目(60071001)、教育部“高等学校骨干教师”资助计划(2000)和东南大学航科基金(1999)资助项目.
作者简介: 王轶文(1978—), 女, 硕士生; 何农跃(联系人), 男,博士, 教授, 博士生导师, nyhe@seu.edu.cn.
更新日期/Last Update: 2002-01-20