[1]马明,朱毅,张宇,等.四氧化三铁纳米粒子与癌细胞相互作用的初步研究[J].东南大学学报(自然科学版),2003,33(2):205-207.[doi:10.3969/j.issn.1001-0505.2003.02.022]
 Ma Ming,Zhu Yi,Zhang Yu,et al.Preparation of magnetite nanoparticles and interaction with cancer cells[J].Journal of Southeast University (Natural Science Edition),2003,33(2):205-207.[doi:10.3969/j.issn.1001-0505.2003.02.022]
点击复制

四氧化三铁纳米粒子与癌细胞相互作用的初步研究()
分享到:

《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
33
期数:
2003年第2期
页码:
205-207
栏目:
生物医学工程
出版日期:
2003-03-20

文章信息/Info

Title:
Preparation of magnetite nanoparticles and interaction with cancer cells
作者:
马明 朱毅 张宇 张东生 唐祖明 张海黔 顾宁
东南大学生物科学与医学工程系,南京 210096
Author(s):
Ma Ming Zhu Yi Zhang Yu Zhang Dongsheng Tang Zuming Zhang Haiqian Gu Ning
Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
关键词:
Fe3O4 纳米粒子 癌细胞
Keywords:
magnetite nanoparticles cancer cell
分类号:
Q26
DOI:
10.3969/j.issn.1001-0505.2003.02.022
摘要:
用共沉淀法制备了粒径为7.5 nm的Fe3O4纳米粒子,并用透射电镜(TEM)观察其形貌为分布均匀的球形.将Fe3O4纳米粒子加入癌细胞株7901和MKN-45的培养液中,培养一段时间后,通过TEM观察细胞的形貌,发现Fe3O4纳米粒子被摄入到癌细胞内,通过原子吸收光谱(AAS)测量了细胞对Fe3O4纳米粒子的摄入量.结果表明,Fe3O4纳米粒子可以逐渐被癌细胞摄入,在癌细胞内达到一定的浓度范围.该研究为利用Fe3O4纳米粒子的磁过热疗法治疗肿瘤提供了细胞层次的实验和理论基础.
Abstract:
Magnetite nanoparticles with mean diameter of 7.5 nm were prepared by co-precipitation method. The morphology of particles was observed by using TEM. The obtained magnetite nanoparticles were added into the culture media of cancer cell lines 7901 and MKN-45. The cells’ morphology was observed through TEM. The particles intracellular uptake was measured by atom absorb spectrum(AAS). The results indicate that Fe3O4 nanoparticles can be taken into tumor cells at some concentrations. The study provides theoretic and experimental support for magnetic hyperthermia on tumor.

参考文献/References:

[1] Hai Y,Pai V,Chen C J.Development of magnetic device for cell separation [J].J Magn Magn Mater,1999,194(1-3):254-261.
[2] Chen D,Liao M.Preparation and characterization of YADH-bound magnetic nanoparticles[J].J Molecular Catalysis,2002,16(5/6):283-291.
[3] 常津.具有负荷靶向抗癌功能的纳米高分子材料——阿霉素免疫磁性毫微粒的制备及体外试验[J].中国生物医学工程学报,1996,15(3):97-101.
  Chang Jin.Preparation of B1-target anticancer drugs — immunological magnetic adriamycin nanoparticles and its evaluation in vitro[J].Chinese J Biomed Eng,1996,15(3):97-101.(in Chinese)
[4] Torchilin V P.Drug targeting[J].Europ J Pharmaceutical Sci,2000,11(2):81-91.
[5] Reimer P,Weissleder R.Development and experimental application of receptor-specific MR contrast media[J].Der Radiologe,1996,36(2):153.
[6] Chouly C,Pouliquen D,Lucet I,et al.Development of superparamagnetic nanoparticles for MRI:effect of particle size,charge and surface nature on biodistribution [J].J Microencapsulation,1996,13(3):245-255.
[7] Chan D C F,Kirpotin D,Bunn P A.Synthesis and evaluation of colloidal magnetic iron-oxides for the site-specific radiofrequency induced hyperthermia of cancer[J].J Magn Magn Mater,1993,122(8):374-379.
[8] Jordan A,Wust P,Scholz R,et al. Scientific and clinical applications of magnetic carriers[M].New York:Plenum Press,1997.569-575.
[9] Mornet S,Vekris A,Bonnet J,et al.DNA-magnetite nanocomposite materials[J].Mater Leter,2000,42(3):183-188.
[10] 邱星屏.Fe3O4磁性纳米粒子的合成及表征[J].厦门大学学报(自然科学版),1999,38(5):711-715.
  Qiu Xingping.Preparation and characterization of Fe3O4,magnetic nanoparticles [J]. Journal of Xiamen University(Natural Science Edition),1999, 38(5):711-715.(in Chinese)

相似文献/References:

[1]段炼,韩吉田,霍冲,等.热电制冷液冷服内纳米流体自然循环换热特性[J].东南大学学报(自然科学版),2018,48(2):220.[doi:10.3969/j.issn.1001-0505.2018.02.005]
 Duan Lian,Han Jitian,Huo Chong,et al.Natural circulation heat transfer characteristics of nanofluid in thermoelectric refrigeration liquid cooling suits[J].Journal of Southeast University (Natural Science Edition),2018,48(2):220.[doi:10.3969/j.issn.1001-0505.2018.02.005]

备注/Memo

备注/Memo:
基金项目: 江苏省高技术研究项目(BG2001006)、国家高科技研究发展计划(2002AA302207).
作者简介: 马明(1975—),女,博士生; 顾宁(联系人),男,博士,教授,博士生导师,guning@seu.edu.cn.
更新日期/Last Update: 2003-03-20