[1]李萍,戎非,谢一兵,等.(S)-萘普生分子印迹聚合物结合特性及制备中复合物的性能研究[J].东南大学学报(自然科学版),2003,33(4):475-478.[doi:10.3969/j.issn.1001-0505.2003.04.023]
 Li Ping,Rong Fei,Xie Yibing,et al.Study on binding characteristics of(S)-naproxen imprinted polymer and performance of its complexes during preparation[J].Journal of Southeast University (Natural Science Edition),2003,33(4):475-478.[doi:10.3969/j.issn.1001-0505.2003.04.023]
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(S)-萘普生分子印迹聚合物结合特性及制备中复合物的性能研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
33
期数:
2003年第4期
页码:
475-478
栏目:
化学化工
出版日期:
2003-07-20

文章信息/Info

Title:
Study on binding characteristics of(S)-naproxen imprinted polymer and performance of its complexes during preparation
作者:
李萍12 戎非1 谢一兵1 朱馨乐1 袁春伟1
1 东南大学生物科学与医学工程系, 南京 210096; 2 北京航空航天大学生物工程系,北京 100083
Author(s):
Li Ping Rong Fei Xie Yibing Zhu Xinle Yuan Chunwei
1 Department of Biomedical Science and Medical Engineering, Southeast University, Nanjing 210096, China
2 Department of Bioengineering, Beijing University of Aeronautics and Astronautics, Beijng 100083, China
关键词:
(S)-萘普生 分子印迹聚合物 结构模拟 结合位点
Keywords:
(S)-naproxen molecularly imprinted polymer structure simulation binding sites
分类号:
O658.1
DOI:
10.3969/j.issn.1001-0505.2003.04.023
摘要:
以丙烯酰胺为功能单体,合成了对(S)-萘普生具有高手性选择性的分子印迹聚合物.通过紫外光谱分析了聚合物制备过程中(S)-萘普生与功能单体丙烯酰胺形成的复合物,并用Hyperchem软件模拟其结构.分子印迹聚合物对模板分子的结合量高于其对映异构体,手性分离因子α达1.87.Scatchard分析表明分子印迹聚合物在识别(S)-萘普生分子过程中存在2类结合位点,计算得到高亲和力的结合位点的离解常数Kd,1和最大表观结合常数Qmax,1分别为28.0 μmol/L 和 52.94 μmol/g,低亲和力的结合位点的离解常数Kd,2和最大表观结合常数Qmax,2max,2分别为0.489 mmol/L 和0.122 mmol/g.
Abstract:
A molecularly imprinted polymer(MIP)was synthesized as the highly chiral selective materials for(S)-naproxen with acrylamide as functional monomers. UV(ultra-violet)studies showed that the template(S)-naproxen and functional monomer acrylamide formed complexes before polymerization and Hyperchem was used to simulate the structures of complexes. The binding capacity of MIP to(S)-naproxen is higher than that of its enantiomer and the chiral separation factor α is 1.87. Scatchard analysis suggests the MIP recognizing(S)-naproxen with two classes of binding sites. The calculated dissociation constant Kd,1 and apparent maximum number Qmax,1max,1 of binding sites with high affinity are 28.0 μmol/L and 52.94 μmol/g respectively, while Kd,2 and Qmax,2max,2 of binding sites with low affinity are 0.489 mmol/L and 0.122 mmol/g.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 高校博士学科点专项科研基金资助项目(20010286006)、江苏省自然科学基金资助项目(BK2001015).
作者简介: 李萍(1974—),女,博士生; 袁春伟(联系人),男,教授,博士生导师,cwy@seu.edu.cn.
更新日期/Last Update: 2003-07-20