[1]段磊,夏强,马全红,等.维甲酸纳米颗粒混悬剂的制备及表征[J].东南大学学报(自然科学版),2004,34(3):344-347.[doi:10.3969/j.issn.1001-0505.2004.03.014]
 Duan Lei,Xia Qiang,Ma Quanhong,et al.Preparation and characterization of retinoic acid nanoparticle suspension[J].Journal of Southeast University (Natural Science Edition),2004,34(3):344-347.[doi:10.3969/j.issn.1001-0505.2004.03.014]
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维甲酸纳米颗粒混悬剂的制备及表征()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
34
期数:
2004年第3期
页码:
344-347
栏目:
生物医学工程
出版日期:
2004-05-20

文章信息/Info

Title:
Preparation and characterization of retinoic acid nanoparticle suspension
作者:
段磊 夏强 马全红 张晓佳 顾宁
东南大学纳米科学与技术研究中心, 南京 210096
Author(s):
Duan Lei Xia Qiang Ma Quanhong Zhang Xiaojia Gu Ning
Research Center for Nano-scale Science and Technology, Southeast University, Nanjing 210096, China
关键词:
维甲酸 纳米颗粒混悬剂 PVR
Keywords:
retinoic acid nanoparticle suspension proliferative vitreoretinopathy(PVR)
分类号:
R9;TQ46
DOI:
10.3969/j.issn.1001-0505.2004.03.014
摘要:
采用溶剂分散法制备了不同药物质量浓度的维甲酸纳米颗粒混悬剂,并对其进行了冷冻干燥; 采用光子相关谱(PCS)测定了冷冻干燥前后混悬剂中纳米粒子的平均粒径; 透射电镜(TEM)观察其微观形貌; 高效液相色谱法(HPLC)对不同时间混悬剂中全反式维甲酸的质量浓度进行定量测定.结果表明,混悬剂中维甲酸纳米粒子(RA-NP)的粒径保持在200~400 nm之间,大都为完整的球形; 与维甲酸硅油10~15 mg/L的药物质量浓度相比,纳米颗粒混悬剂中有效药物的质量浓度可显著提高至35 mg/L; 药物降解速度得到明显降低,4周之内能维持抑制细胞增殖的有效药物质量浓度.维甲酸纳米颗粒混悬剂有望成为一种新型的增殖性玻璃体视网膜病变(PVR)治疗用药物载体.
Abstract:
Retinoic acid(RA)nanoparticle suspensions with different concentrations were prepared by solvent-diffusion method in aqueous and freeze-drying. The mean particle sizes before and after drying were determined by photon correlation spectroscopy method and the morphology of RA nanoparticles was characterized by transmission electron microscope. The concentrations of all-trans RA(ATRA)in different time were measured by high performance liquid chromatography method to analyze drug loading and stability. The results show that the mean diameter of RA nanoparticles(RA-NP)falls into 200-400 nm. Drug loading of the system is 35 mg/L. It is demonstrated that the concentration of ATRA can be kept in the range of effective antiproliferation for 4 weeks, which indicates that RA nanoparticle suspension has effectively prolonged drug release. This research demonstrates that RA nanoparticle suspension can be a very potential drug carrier to prevent proliferative vitreoretinopathy(PVR).

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备注/Memo

备注/Memo:
基金项目: 江苏省发展计划委员会与人事部留学回国人员资助项目(7607034018).
作者简介: 段磊(1979—),女,硕士生; 顾宁(联系人),男,博士,教授,博士生导师,guning@seu.edu.cn.
更新日期/Last Update: 2004-05-20