[1]覃玉荣,赖声礼.细胞悬液中细胞跨膜电位计算模型[J].东南大学学报(自然科学版),2004,34(5):661-664.[doi:10.3969/j.issn.1001-0505.2004.05.022]
 Qin Yurong,Lai Shengli.Analytical model for transmembrane potential induced on a cell membrane in suspensions[J].Journal of Southeast University (Natural Science Edition),2004,34(5):661-664.[doi:10.3969/j.issn.1001-0505.2004.05.022]
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细胞悬液中细胞跨膜电位计算模型()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
34
期数:
2004年第5期
页码:
661-664
栏目:
生物医学工程
出版日期:
2004-09-20

文章信息/Info

Title:
Analytical model for transmembrane potential induced on a cell membrane in suspensions
作者:
覃玉荣 赖声礼
华南理工大学电子与信息学院, 广州 510641
Author(s):
Qin Yurong Lai Shengli
College of Electronics and Information Engineering, South China University of Technology, Guangzhou 510641, China
关键词:
细胞悬液 跨膜电位 计算模型 电场 生物效应
Keywords:
cell suspensions transmembrane potential analytical model electric field biological effects
分类号:
R815
DOI:
10.3969/j.issn.1001-0505.2004.05.022
摘要:
由于建立外加电场中悬液细胞跨膜电位计算模型非常困难,本文用近似场等效方法解决了该建模难题.首先用Maxwell等效原理,计算处于外加场中细胞悬液的平均场.然后确定悬液中一个细胞局部的实际场分布和独立处于外加场中单细胞场分布近似等效的条件,再基于单细胞膜电位求解方法,建立反映均匀排列悬液细胞跨膜电位变化的计算模型.模型表明了细胞膜电位的变化和外加场、细胞种类、细胞浓度以及细胞排列方式的关系,揭示了悬液细胞相对单细胞跨膜电位下降的物理过程.最后用该模型计算了悬液中细胞跨膜电位并和数值解进行比较,结果基本符合.
Abstract:
Since the analytical model for TMP(transmembrane potential)on suspension cells exposed to external electric field is too complex to obtain, an approximate equivalence method is proposed to resolve this problem. First, the average field inside the cell suspensions is calculated according to the Maxwell equivalence principle. Then the condition, under which the local electric field of unit cell in suspensions is approximately similar to that of a single cell exposed to external field, is investigated. Based on the analytical solution of single cell, an analytical model for the TMP on the suspension cells is built. The model shows that the TMP depends on parameters such as the external field, the cell arrangement and the cell volume fraction. Finally, the TMP on a suspension cell is calculated using the proposed analytical model and the results are compared with those from numerical calculation using finite-element method. It is shown that the analytical results are in agreement with the numerical results.

参考文献/References:

[1] Tsong T Y. Electroporation of cell membrane [J]. Biophysical Journal, 1991,60:297-306.
[2] Kotnik T,Miklavcic D.Analytical description of tansmembrane voltage induced by electric field on spherical cells[J].Biophysical Journal,2000,79(2):670-679.
[3] Pavlin M,Slivnik T,Miklavcic D.Effective conductivity of cell suspensions [J].IEEE Transactions on Biomedical Engineering, 2002,49(1):77-80.
[4] Heida T,Wagenaar J B M,Rutten W L C,et al.Investigating membrane breakdown of neuronal cells exposed to nonuniform electric field by finite-element modeling and experiments [J].IEEE Transactions on Biomedical Engineering, 2002,49(10):1195-1203.
[5] Pavlin M,Pavselj N,Miklavcic D.Dependence of induced transmembrane potential on cell density,arrangement,and cell position inside a cell system[J].IEEE Transactions on Biomedical Engineering, 2002,49(6):605-612.
[6] Weave J C.Electroporation of cells and tissues [J]. IEEE Transactions on Plasma Science,2000,28(1):24-33.

备注/Memo

备注/Memo:
基金项目: 国家自然科学基金资助项目(60071033)、广东省自然科学基金资助项目(2002C30202).
作者简介: 覃玉荣(1965—),女,博士生,qyr111@163.com; 赖声礼(联系人),男,教授,博士生导师,Soka@263.net.
更新日期/Last Update: 2004-09-20