[1]纪荣平,李先宁,吕锡武,等.人工介质富集微生物对藻类和藻毒素降解试验研究[J].东南大学学报(自然科学版),2005,35(3):442-445.[doi:10.3969/j.issn.1001-0505.2005.03.025]
 Ji Rongping,Li Xianning,Lü Xiwu,et al.Study on biological degradation of algae and microcystins by means of enrichment microbes on artificial medium[J].Journal of Southeast University (Natural Science Edition),2005,35(3):442-445.[doi:10.3969/j.issn.1001-0505.2005.03.025]
点击复制

人工介质富集微生物对藻类和藻毒素降解试验研究()
分享到:

《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
35
期数:
2005年第3期
页码:
442-445
栏目:
环境科学与工程
出版日期:
2005-05-20

文章信息/Info

Title:
Study on biological degradation of algae and microcystins by means of enrichment microbes on artificial medium
作者:
纪荣平1 李先宁1 吕锡武1 朱光灿1 浦跃朴2 张立将2 赵传鹏2
1 东南大学土木工程学院, 南京 210096; 2 东南大学公共卫生学院, 南京 210009
Author(s):
Ji Rongping1 Li Xianning1 Lü Xiwu1 Zhu Guangcan1Pu Yuepu2 Zhang Lijiang2 Zhao Chuanpeng2
1 College of Civil Engineering, Southeast University, Nanjing 210096, China
2 School of Public Health, Southeast University, Nanjing 210009, China
关键词:
人工介质 生物降解 微囊藻毒素 溶藻细菌 假单胞菌属 芽孢杆菌属
Keywords:
artificial medium biological degradation microcystins algae-lysing bacteria Bacillus spp. Pseudomonas.spp.
分类号:
X172
DOI:
10.3969/j.issn.1001-0505.2005.03.025
摘要:
采用人工介质富集微生物的方法对藻类和微囊藻毒素的生物降解进行了试验研究.中试结果表明:在水力停留时间6~7 d,源水叶绿素a为15.3~266.1 μg/L条件下,人工介质对叶绿素a的去除率达59.4%.运用高效液相色谱(HPLC)对微囊藻毒素进行了检测,当进水总微囊藻毒素TMC-RR和TMC-LR分别为0.25~8.93,0.15~4.73 μg/L,胞外微囊藻毒素EMC-RR和EMC-LR分别为0.13~0.68,0.02~0.11 μg/L时,人工介质对TMC-RR,TMC-LR和EMC-RR,EMC-LR平均去除率分别为69.8%,93.7%,42.2%和68.4%.聚合酶链反应(PCR)电泳图谱发现,人工介质上富集有大量的假单胞菌属和芽孢杆菌属等溶藻细菌.通过人工介质富集微生物的方法可有效降解太湖水体中的藻类和微囊藻毒素.
Abstract:
Biological degradation of algae and microcystins(MCs)through the use of enrichment microbes by artificial medium is studied. The results show that when chl-a in Taihu Lake is 15.3-266.1 μg/L, 59.4% of Chl-a can be removed within 6-7 d. High performance liquid chromatography(HPLC)analysis for detection of MCs is applied. When total microcystin RR and LR(TMC-RR and TMC-LR),extracellular microcystin RR and LR(EMC-RR and EMC-LR)are 0.25-8.93,0.15-4.73,0.13-0.68,0.02-0.11 μg/L respectively in source water, the average removal rates of TMC-RR,TMC-LR,EMC-RR,EMC-LR are 69.8%,93.7%,42.2% and 68.4% respectively. Polymerase chain reaction(PCR)electrophoresis chart shows that there are a great amount of algae-lysing bacteria as Bacillus spp.and Pseudomonas spp. on the artificial medium. Algae and microcystin in the Taihu Lake can be effectively degraded by enrichment microbes on the artificial medium.

参考文献/References:

[1] Chow C W K,Drikas M,House J,et al.The impact of convertional water treatment process on cells of the cyanobacterium Microcystis aeruginosa[J].Wat Res,1999,33(15):3253-3262.
[2] Tsuji K T,Watanuki T.Stability of microcystins from cyanobacteria-Ⅳ.Effect of chlorination on decomposition[J].Toxicon,1997,35(7):1033-1041.
[3] 吕锡武,稻森悠平,丁国际.有毒蓝藻及藻毒素生物降解的初步研究[J].中国环境科学,1999,19(2):138-140.
  Lü Xiwu,Yuhei Inamori,Ding Guoji.Degradation of microcystis viridis and microcystins with biological reactors[J].China Environmental Science,1999,19(2):138-140.(in Chinese)
[4] 彭超,吴刚,席宇,等.3株溶藻细菌的分离鉴定及其溶藻效应[J].环境科学研究,2003,16(1):37-40.
  Peng Chao,Wu Gang,Xi Yu,et al.Isolation and identification of three algae-lysing bacteria and their lytic effects on blue-green algae(Cyanobacteria)[J].Research of Environmental Sciences,2003,16(1):37-40.(in Chinese)
[5] 郭亚新,程凯,赵以军,等.淡水噬藻体及其他溶藻因子的分布与感染力[J].中国环境科学,2003,23(2):167-170.
  Guo Yaxin,Cheng Kai,Zhao Yijun,et al.The distribution and infectivity of cyanophage and other algae-lysin factors in fresh water[J].China Environmental Science,2003,23(2):167-170.(in Chinese)
[6] Takenaka S,Watanabe M F.Microcystin LR degradation by Pseudomonas aeruginosa alkaline protease[J].Chemosphere,1997,34(4):749-757.
[7] Miller M J,Fallowfield H J.Degradation of cyanobacterial hepatotoxins in batch experiments[J].Wat Sci Tech,2001,43(12):229-232.
[8] Cousins I T,Bealing D J,James H A,et al.Biodegradation of microcystin-LR by indigenous mixed bacterial populations[J].Wat Res,1996,30(2):481-485.
[9] 金丽娜,张维昊,郑利,等.滇池水环境中微囊藻毒素的生物降解[J].中国环境科学,2002,22(2):189-192.
  Jin Lina,Zhang Weihao,Zheng Li,et al.Biodegradation of microcystin in Dianchi Lake aquatic environment[J].China Environmental Science, 2002,22(2):189-192.(in Chinese)
[10] Meriluoto J.Chromatography of microcystins[J].Analytica Chimica Acta,1997,352:277-298.
[11] Lahti K,Rapala J,Fardig M,et al.Persistence of cyanobacterial hepatotoxin,microcystin-LR particulate material and dissolved in lake water[J].Wat Res,1997,31(5):1005-1012.
[12] Welker M,Steinberg C.Indirect photolysis of cyanotoxins:one possible mechanism for their low persistence[J].Wat Res,1999,33(5):1159-1164.

相似文献/References:

[1]于光,李云晖,姚琛,等.聚己内酯纳米纤维膜固定化溶藻菌对藻类和藻毒素的生物降解作用[J].东南大学学报(自然科学版),2008,38(3):483.[doi:10.3969/j.issn.1001-0505.2008.03.023]
 Yu Guang,Li Yunhui,Yao Chen,et al.Biological degradation of algae and microcystins by algicidal bacteria immobilized on nanofiber membrane of polycaprolactone[J].Journal of Southeast University (Natural Science Edition),2008,38(3):483.[doi:10.3969/j.issn.1001-0505.2008.03.023]

备注/Memo

备注/Memo:
基金项目: 国家高技术研究发展计划(863计划)资助项目(2002AA601011).
作者简介: 纪荣平(1965—),男,博士生,副教授; 吕锡武(联系人),男,博士,教授,博士生导师,xiwulu@public1.ptt.js.cn.
更新日期/Last Update: 2005-05-20