[1]戎非,李萍,袁春伟.帕珠沙星分子印迹聚合物结合特性[J].东南大学学报(自然科学版),2005,35(5):801-804.[doi:10.3969/j.issn.1001-0505.2005.05.031]
 Rong Fei,Li Ping,Yuan Chunwei.Binding characteristics of molecularly imprinted polymer for pazufloxacin[J].Journal of Southeast University (Natural Science Edition),2005,35(5):801-804.[doi:10.3969/j.issn.1001-0505.2005.05.031]
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帕珠沙星分子印迹聚合物结合特性()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
35
期数:
2005年第5期
页码:
801-804
栏目:
化学化工
出版日期:
2005-09-20

文章信息/Info

Title:
Binding characteristics of molecularly imprinted polymer for pazufloxacin
作者:
戎非1 李萍2 袁春伟1
1 东南大学生物科学与医学工程系, 南京 210096; 2 北京航空航天大学生物工程系, 北京 100083
Author(s):
Rong Fei1 Li Ping2 Yuan Chunwei1
1 Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
2 Department of Bioengineering, Beijing University of Aeronautics and Astronautics, Beijing 100083, China
关键词:
帕珠沙星 分子印迹聚合物 Scatchard分析 结合位点
Keywords:
pazufloxacin molecularly imprinted polymer Scatchard analysis binding sites
分类号:
O631.3
DOI:
10.3969/j.issn.1001-0505.2005.05.031
摘要:
采用甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,合成了具有高选择性的L-帕珠沙星分子印迹聚合物.印迹聚合物对模板分子的结合量明显高于空白聚合物.通过Scatchard分析研究了聚合物的选择结合性能,结果表明:分子印迹聚合物在识别帕珠沙星分子的过程中存在两类结合位点; 用多点结合模型进行计算得到高亲和力的结合位点的离解常数Kd,1和最大表观结合常数Qmax,1max,1分别为23.08 μmol/L和3.65 μmol/g,低亲和力的结合位点的离解常数Kd,2和最大表观结合常数Qmax,2max,2分别为0.43 mmol/L和27.28 μmol/g.实验表明,分子印迹聚合物对模板分子的结合量高于其他类似物,呈现出空白聚合物没有的高选择性.
Abstract:
A molecularly imprinted polymer(MIP)with high selectivity was prepared using methacrylic acid(MAA)as monomer, ethylene glycol dimethacrylate(EDMA)as cross-linker and L-pazufloxacin as template. The binding capacity of MIP to the template is higher than that of blank polymer. Scatchard analysis show that the MIP recognized template with two classes of binding sites. The multiple-sites binding model was used to calculate the dissociation constant Kd and apparent maximum number Qmaxmax of binding sites. Kd,1 and Qmax,1 of the binding sites with high affinity are 23.08 μmol/L and 3.65 μmol/g, while Kd,2 and Qmax,2 of the binding sites with low affinity are 0.43 mmol/L and 27.28 μmol/g respectively. The selective binding experiment for substrates shows that the affinity and selectivity for pazufloxacin are higher than that for L-norfloxacin and ofloxacin.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 高等学校博士学科点专项基金资助项目(20010286006)、江苏省自然科学基金资助项目(BK2001015).
作者简介: 戎非(1977—),男,博士生; 袁春伟(联系人),男,教授,博士生导师, cwy@seu.edu.cn.
更新日期/Last Update: 2005-09-20