[1]赵传鹏,浦跃朴,尹立红,等.实时荧光定量PCR法检测环境假单胞菌属细菌丰度[J].东南大学学报(自然科学版),2006,36(1):143-146.[doi:10.3969/j.issn.1001-0505.2006.01.029]
　Zhao Chuanpeng,Pu Yuepu,Yin Lihong,et al.Detection of abundance of Pseudomonas in environmental samples by real-time quantitative PCR[J].Journal of Southeast University (Natural Science Edition),2006,36(1):143-146.[doi:10.3969/j.issn.1001-0505.2006.01.029]
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实时荧光定量PCR法检测环境假单胞菌属细菌丰度()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
36
期数:
2006年第1期
页码:
143-146
栏目:
环境科学与工程
出版日期:
2006-01-20

文章信息/Info

Title:
Detection of abundance of Pseudomonas in environmental samples by real-time quantitative PCR
作者:
赵传鹏1;  浦跃朴1;  尹立红1;  梁戈玉1;  吕锡武2;  李先宁2
1 东南大学公共卫生学院, 南京 210009; 2 东南大学土木工程学院, 南京 210096
Author(s):
Zhao Chuanpeng1;  Pu Yuepu1;  Yin Lihong1;  Liang Geyu1;  Lü Xiwu2;  Li Xianning2
1 School of Public Health, Southeast University, Nanjing 210009, China
2 College of Civil Engineering, Southeast University, Nanjing 210096, China
关键词:
实时荧光定量PCR;  假单胞菌属;  人工介质;  溶藻细菌
Keywords:
real-time quantitative PCR;  Pseudomonas;  artificial mediums;  algicidal bacteria
分类号:
X172
DOI:
10.3969/j.issn.1001-0505.2006.01.029
摘要:
应用实时荧光定量PCR法,建立了总细菌及假单胞菌属的标准曲线,并以环境样本中假单胞菌属与总细菌的比值反映其细菌丰度.应用该方法评价人工除藻反应器中组合填料、无纺布、弹性填料等介质对太湖水中溶藻细菌之一的假单胞菌属的富集情况.结果显示:该方法对总细菌的检测范围为103～108个基因拷贝/μL(R2=0.997); 假单胞菌属的检测范围为1～105个基因拷贝/μL(R2=0.994).对除藻反应器溶藻细菌富集效果的评价显示,所建立的方法能有效检测反应器中填料对该类溶藻细菌的富集程度.初步证明所建立的方法可有效定量假单胞菌属在环境样本中的丰度.
Abstract:
A real-time quantitative PCR(polymerase chain reaction)(RTQ-PCR)method is established to quantitatively detect the abundance of algicidal bacteria of Pseudomonas in environmental samples in this study. The standard abundance curves of total bacteria and Pseudomonas were established respectively and the abundance of Pseudomonas was demonstrated by the Ct ratio of Pseudomonas and total bacteria. The abundance of algicidal bacteria of Pseudomonas on the artificial mediums of algae treatment reactor in Taihu Lake were then detected by the method. The results show that the determination ranges of total bacteria and Pseudomonas are 103-108gene copies/μL(R2=0.997)and 1-105 gene copies/μL(R2=0.994), respectively. The accumulation of Pseudomonas on the artificial mediums in reactor can be found effectively by the assay. It is believed that RTQ-PCR method can detect the abundance of algicidal bacteria of Pseudomonas in environmental samples quantitatively.

参考文献/References:

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相似文献/References:

[1]赵传鹏,浦跃朴,尹立红,等.溶微囊藻菌的分离与溶藻作用[J].东南大学学报(自然科学版),2005,35(4):602.[doi:10.3969/j.issn.1001-0505.2005.04.023]
　Zhao Chuanpeng,Pu Yuepu,Yin Lihong,et al.Isolation and algicidal effect of a lytic Microcystis bacterium from Taihu Lake[J].Journal of Southeast University (Natural Science Edition),2005,35(1):602.[doi:10.3969/j.issn.1001-0505.2005.04.023]
[2]纪荣平,李先宁,吕锡武,等.人工介质富集微生物对藻类和藻毒素降解试验研究[J].东南大学学报(自然科学版),2005,35(3):442.[doi:10.3969/j.issn.1001-0505.2005.03.025]
　Ji Rongping,Li Xianning,Lü Xiwu,et al.Study on biological degradation of algae and microcystins by means of enrichment microbes on artificial medium[J].Journal of Southeast University (Natural Science Edition),2005,35(1):442.[doi:10.3969/j.issn.1001-0505.2005.03.025]

备注/Memo

备注/Memo:
基金项目: 国家高技术研究发展计划(863计划)资助项目(2002AA601011)、江苏省自然科学基金资助项目(BK2005068)、教育部博士点专项科研基金资助项目(20050286035).
作者简介: 赵传鹏(1978—),男,硕士生; 浦跃朴(联系人),男,博士,教授,博士生导师, yppu@seu.edu.cn.
更新日期/Last Update: 2006-01-20