[1]蒋犁,余章斌,张春秀,等.不同方法修饰玻璃表面制备蛋白质芯片的对比[J].东南大学学报(自然科学版),2007,37(1):136-140.[doi:10.3969/j.issn.1001-0505.2007.01.029]
 Jiang Li,Yu Zhangbin,Zhang Chunxiu,et al.Comparison between different modificative strategies of glass surfaces to prepare protein microarrays[J].Journal of Southeast University (Natural Science Edition),2007,37(1):136-140.[doi:10.3969/j.issn.1001-0505.2007.01.029]
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不同方法修饰玻璃表面制备蛋白质芯片的对比()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
37
期数:
2007年第1期
页码:
136-140
栏目:
生物医学工程
出版日期:
2007-01-20

文章信息/Info

Title:
Comparison between different modificative strategies of glass surfaces to prepare protein microarrays
作者:
蒋犁1 余章斌13 张春秀2 唐祖明2 陆祖宏2 蒋涛3
1 东南大学临床医学院, 南京 210009; 2 东南大学生物电子学国家重点实验室, 南京 210096; 3 南京市妇幼保健院, 南京 210004
Author(s):
Jiang Li1 Yu Zhangbin13 Zhang Chunxiu2 Tang Zuming2 Lu Zuhong2 Jiang Tao3
1 School of Clinical Medicine, Southeast University, Nanjing 210009, China
2 State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
3 Nanjing Maternity and Child Health Hospital, Nanjin
关键词:
蛋白质芯片 氨基修饰 戊二醛修饰 聚赖氨酸修饰 聚丙烯酰胺修饰 琼脂糖修饰
Keywords:
protein microarray aminosilane modification glutaraldehyde modification poly-L-lysine modification polyacrylamide modification agarose modification
分类号:
R394.3
DOI:
10.3969/j.issn.1001-0505.2007.01.029
摘要:
分别采用3-氨基丙基-三甲氧基硅烷、戊二醛、多聚赖氨酸、聚丙烯酰胺、琼脂糖、硝化纤维修饰玻璃表面,对6种不同修饰表面制备的蛋白质芯片进行对比研究,探讨制备蛋白质芯片的合适玻璃表面修饰方法.利用原子力学显微镜对其表面进行表征,通过点样蛋白于修饰后的玻片表面制备蛋白质芯片,比较不同修饰表面对蛋白质的固定效率和固定效果.结果发现:氨基、醛基、聚赖氨酸修饰玻片为二维平面结构,琼脂糖、聚丙烯酰胺、硝化纤维修饰玻片其表面不仅有多孔结构,而且有一定厚度; 琼脂糖修饰玻片所得的荧光强度最高,背景较低,是一种理想的制备蛋白质芯片的表面修饰方法.
Abstract:
Glass slides were modified with 3-aminopropyltriethoxysilane, glutaraldehyde, poly-L-lysine, polyacrylamide, agarose film, and nitrocellulose to prepare protein microarrays.Atomic force microscopy was used to obtain three-dimensional surface profiles of the modified glass surfaces.The efficiency and the effects of immobilized proteins on the glass were compared. The results indicate that aminosilane, glutaraldehyde, and poly-L-lysine coated glass slides show two-dimensional surfaces; while glass slides modified with polyacrylamide, agarose, and nitrocellulose possess more holes and greater thickness. Agarose film coated glass slides demonstrate highest immobilization capacity, and lowest background, which is more suitable for preparing protein microarray.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 江苏省自然科学基金资助项目(BK2003056).
作者简介: 蒋犁(1953—),女,教授,Jiangli7@sohu.com.
更新日期/Last Update: 2007-01-20