[1]于光,李云晖,姚琛,等.聚己内酯纳米纤维膜固定化溶藻菌对藻类和藻毒素的生物降解作用[J].东南大学学报(自然科学版),2008,38(3):483-487.[doi:10.3969/j.issn.1001-0505.2008.03.023]
　Yu Guang,Li Yunhui,Yao Chen,et al.Biological degradation of algae and microcystins by algicidal bacteria immobilized on nanofiber membrane of polycaprolactone[J].Journal of Southeast University (Natural Science Edition),2008,38(3):483-487.[doi:10.3969/j.issn.1001-0505.2008.03.023]
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聚己内酯纳米纤维膜固定化溶藻菌对藻类和藻毒素的生物降解作用()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
38
期数:
2008年第3期
页码:
483-487
栏目:
环境科学与工程
出版日期:
2008-05-20

文章信息/Info

Title:
Biological degradation of algae and microcystins by algicidal bacteria immobilized on nanofiber membrane of polycaprolactone
作者:
于光1;  李云晖1;  姚琛2;  尹立红1;  李新松2;  浦跃朴1
1 东南大学公共卫生学院, 南京 210009; 2 东南大学化学化工学院, 南京 210096
Author(s):
Yu Guang1;  Li Yunhui1;  Yao Chen2;  Yin Lihong1;  Li Xinsong2;  Pu Yuepu1
1 School of Public Health, Southeast University, Nanjing 210009, China
2 School of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, China
关键词:
溶藻菌;  人工介质;  固定化;  生物降解;  微囊藻毒素LR
Keywords:
algicidal bacteria;  artificial medium;  immobilization;  biological degradation;  microcystins-LR
分类号:
X172
DOI:
10.3969/j.issn.1001-0505.2008.03.023
摘要:
采用电纺法制备并表征了聚己内酯纳米纤维膜(NMP).将从太湖水体中分离到的假单胞菌属溶藻菌固定化在聚己内酯纳米纤维膜(NMP)、维纶(VNL)和阿科蔓(AQUA)等人工介质材料上,分析与比较不同人工介质固定化溶藻菌对太湖土著铜绿微囊藻和微囊藻毒素LR(MC-LR)的生物降解作用.实验结果显示:NMP吸水率、孔隙率、拉伸强度分别为(81.33±1.58)%、(78.68±4.85)%、(89.8±7.5)MPa.NMP固定化溶藻菌的数量为5.65×107/cm2; NMP固定化溶藻菌对藻细胞的48 h溶藻率和对MC-LR的48 h降解率分别为85.68%和60.16%; 溶解藻细胞可重复利用次数达到4次.上述结果表明,NMP是一种良好的固定化溶藻菌的纳米人工介质,可以增强溶藻菌对铜绿微囊藻和MC-LR的生物降解作用.
Abstract:
Nanofiber membrane of polycaprolactone was prepared by electrospinning and then characterized. The algicidal bacteria of pseudomonas strain isolated from Taihu Lake were then immobilized on three kind of artificial media of NMP(nanofiber membrane polycaprolactone, AQUA(Aquamats)and VNL(Vinlon), respectively. The algae-lytic and microcystin-degradative effects of the immobilized algicidal bacteria were detected and evaluated. The results show that the ratio of water absorptivity, the porosity and the tensile strength of the NMP are(81.33±1.58)%,(78.68±4.85)%,(89.8±7.5)MPa, respectively. The bacteria concentration of 30 h after immobilization on NMP is 5.65×107/cm2. The 48 h algicidal ratio and microcystin-degradative ratio of NMP immobilized algicidal bacteria are 85.68%,60.16% respectively. The utilization of the NMP immobilized algicidal bacteria can be repeated for up to 4 times. NMP is a suitable material for bacteria immobilization by which the effects of immobilized algicidal bacteria on biological degradation of algae and microcystin LR can be improved.

参考文献/References:

[1] 孔繁翔,高光.大型浅水富营养化湖泊中蓝藻水华形成机理的思考[J].生态学报,2005,25(3):589-595.
　　Kong Fanxiang,Gao Guang.Hypothesis on cyanobacteria bloom-forming mechanism in large shallow eutrophic lakes [J].Acta Ecologica Sinica,2005,25(3):589-595.(in Chinese)
[2] Chow C W K,Drikas M,House J,et al.The impact of conventional water treatment process on cells of the cyanobacterium microcystis aeruginosa [J].Water Research,1999,33(15):3253-3262.
[3] Dong Dinjiao,Chen Zhu.Decolourization of reactive brilliant blue KN-R by immobilized cells of aspergillus ficuum [J].Journal of Environmental Science,2003,15(3):377-382.
[4] 赵传鹏,浦跃朴,尹立红,等.溶微囊藻菌的分离与溶藻作用[J].东南大学学报:自然科学版,2005,35(4):602-606.
　 Zhao Chuanpeng,Pu Yuepu,Yin Lihong,et al.Isolation and algicidal effect of a lytic microcystis bacterium from Taihu Lake [J]. Journal of Southeast University:Natural Science Edition,2005,35(4):602-606.(in Chinese)
[5] 郑兴华,高媛.等离子熔射成形件的孔隙率的测定[J].机械工程师,2001(2):41-42.
　 Zheng Xinghua,Gao Yuan.Measurement of porosity of plasma-spray forming parts [J].Mechanical Engineer,2001(2):41-42.(in Chinese)
[6] 孙复钱,李新松.含氟聚合物纳米多孔纤维膜的制备[J].化工新型材料,2006,34(6):12-14.
　 Sun Fuqian,Li Xinsong.Preparation of nano-porous nanofiber mats of fluoro polymers by electrospinning [J].New Chemical Materials,2006,34(6):12-14.(in Chinese)
[7] 裴海燕,胡文容,曲音波,等.一株溶藻细菌的分离鉴定及其溶藻特性[J].环境科学学报,2005,25(6):796-802.
　 Pei Haiyan,Hu Wenrong,Qu Yinbo,et al.Isolation and identification of one algae-lysing bacteria and its lytic character [J]. Acta Scientiae Circumstantiae,2005,25(6):796-802.(in Chinese)
[8] 纪荣平,李先宁,吕锡武,等.人工介质富集微生物对藻类和藻毒素降解试验研究[J].东南大学学报:自然科学版,2005,35(3):442-445.
　 Ji Rongping,Li Xianning,Lü Xiwu,et al.Study on biological degradation of algae and microcystins by means of enrichment microbes on artificial medium [J].Journal of Southeast University:Natural Science Edition,2005,35(3):442-445.(in Chinese)
[9] 宦海琳,韩岚,李建宏,等.五株微囊藻毒素降解菌的分离与鉴定[J].湖泊科学,2006,18(2):184-188.
　 Huan Hailing,Han Lan,Li Jianhong,et al.Isolation and identification of five microcystin degrading bacterial strains [J].J Lake Sci,2006,18(2):184-188.(in Chinese)
[10] 胡文容,李力,刘培启.生物系统固定化强化除藻试验及机理浅析[J].山东工业大学学报,2001,31(6):501-505.
　　Hu Wenrong,Li Li,Liu Peiqi.The analysis of bio-immobilization system algicidal experiment [J]. Journal of Shandong University of Technology,2001,31(6):501-505.(in Chinese)

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备注/Memo

备注/Memo:
作者简介: 于光(1982—),男,硕士生; 浦跃朴(联系人), 男,博士,教授,博士生导师,yppu@seu.edu.cn.
基金项目: 江苏省自然科学基金资助项目(BK2005068)、教育部博士点专项科研基金资助项目(20050286035)、国家重点基础研究发展计划(973计划)资助项目(2006CB705602)、 江苏省“333工程”科研资助项目(苏人才办[2007]16号).
引文格式: 聚己内酯纳米纤维膜固定化溶藻菌对藻类和藻毒素的生物降解作用于光,李云晖,姚琛,等.[J].东南大学学报:自然科学版,2008,38(3):483-487.
更新日期/Last Update: 2008-05-20