[1]杨红,吴秋云,唐萌,等.纳米氧化硅的体外细胞毒性[J].东南大学学报(自然科学版),2008,38(6):1054-1060.[doi:10.3969/j.issn.1001-0505.2008.06.023]
 Yang Hong,Wu Qiuyun,Tang Meng,et al.In vitro study of cytotoxicity of nanosized silica[J].Journal of Southeast University (Natural Science Edition),2008,38(6):1054-1060.[doi:10.3969/j.issn.1001-0505.2008.06.023]
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纳米氧化硅的体外细胞毒性()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
38
期数:
2008年第6期
页码:
1054-1060
栏目:
生物医学工程
出版日期:
2008-11-20

文章信息/Info

Title:
In vitro study of cytotoxicity of nanosized silica
作者:
杨红12 吴秋云2 唐萌2 曹松玉2 董玉颖2 盛静逸2 陆祖宏13
1 东南大学生物电子学国家重点实验室,南京 210096; 2 东南大学公共卫生学院, 南京 210009; 3 东南大学学习科学研究中心, 南京 210096
Author(s):
Yang Hong12 Wu Qiuyun2 Tang Meng2 Cao Songyu2 Dong Yuying2 Sheng Jingyi2 Lu Zuhong13
1 State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096,China
2 School of Public Health, Southeast University, Nanjing 210009,China
3 Research Centre of Learning Science, Southeast University, Nanjing 210096,China
关键词:
纳米氧化硅 红细胞 RAW264.7细胞 毒性效应 氧化应激
Keywords:
nanosized silica erythrocytes RAW264.7 cells toxicity effect oxidative stress
分类号:
R994.3
DOI:
10.3969/j.issn.1001-0505.2008.06.023
摘要:
采用红细胞溶血实验、MTT法、形态学和测定培养液中乳酸脱氢酶(LDH)含量比较纳米SiOx及微米SiO2对红细胞和RAW264.7细胞的毒性作用; 测定丙二醛(MDA)、过氧化氢(H2O2)、羟自由基(·OH)、超氧阴离子自由基(O2·-)、一氧化氮(NO)等氧化应激指标,探讨其毒作用机制.研究结果表明:纳米SiOx与微米SiO2在一定粒子浓度范围内致红细胞溶血率及生成MDA的量随浓度增加逐渐升高,呈剂量-反应关系; RAW264.7细胞的半数抑制浓度(IC50)分别为11.60,38.12 mg/cm2; 染毒细胞体积明显增大,呈气球样变; 纳米SiOx及微米SiO2在0.036~0.571 mg/cm2浓度范围内,可引起培养液中LDH活性显著升高,呈时间-效应关系,相同浓度纳米SiOx致培养液中LDH含量高于微米SiO2; 染毒细胞内MDA,H2O2,·OH,O2·-,NO水平显著升高.结果提示相同浓度纳米SiOx的毒性作用较微米SiO2强,氧化应激在细胞毒性中起一定作用.
Abstract:
The toxicity effects of nanosized silica and micron silicon dioxide were compared by hemolysis test of erythrocytes, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT)assay, cell morphology and detecting lactate dehydrogenase(LDH)in culture medium of RAW264.7 cells. Oxidative stress level indicated by the production of malondialdehyde(MDA), hydrogen peroxide(H2O2), hydroxyl radicals(·OH), superoxide anion radical(O2·-)and nitric oxide(NO)in cells were measured. The results show that after exposure to two kinds of powder within a dose range, the erythrocytes hemolysis rates and concentration of MDA increase in a dose-dependent manner. The inhibitory concentration 50%(IC50)of nanosized silica and micron silicon dioxide is 11.61 and 38.12 mg/cm2 respectively. Meanwhile, ballooning degeneration of cells can be observed by light and fluorescence microscope. The activity of LDH leakage induced by nanosized silica and micron silicon dioxide obviously increases at dosage of 0.036 to 0.571 mg/cm2 which shows a time-effect relationship. The level of LDH leakage caused by nanosized silica is higher than that by micron silicon dioxide at same concentration. The increase of lipid peroxidation product of MDA and free radicals of H2O2,·OH, O2·-and NO of RAW264.7 cells result from nanosized silica and micron silicon dioxide. The effect of cytotoxicity caused by nanosized silica is stronger than that of micron silicon at same dose and the effect of oxidative stress may also be involved.

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备注/Memo

备注/Memo:
作者简介: 杨红(1967—),女,博士生; 陆祖宏(联系人),男,博士,教授,博士生导师,zhlu @seu.edu.cn.
基金项目: 国家重点基础研究发展计划(973计划)资助项目(2006CB705602).
引文格式: 杨红,吴秋云,唐萌,等.纳米氧化硅的体外细胞毒性[J].东南大学学报:自然科学版,2008,38(6):1054-1060.
更新日期/Last Update: 2008-11-20