[1]葛云,章东.活体细胞图像斑点的自动提取和跟踪方法[J].东南大学学报(自然科学版),2009,39(3):464-467.[doi:10.3969/j.issn.1001-0505.2009.03.009]
 Ge Yun,Zhang Dong.Automated methods for particle tracking of the intracellular transport of clathrin-coated pits and vesicles[J].Journal of Southeast University (Natural Science Edition),2009,39(3):464-467.[doi:10.3969/j.issn.1001-0505.2009.03.009]
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活体细胞图像斑点的自动提取和跟踪方法()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
39
期数:
2009年第3期
页码:
464-467
栏目:
计算机科学与工程
出版日期:
2009-05-20

文章信息/Info

Title:
Automated methods for particle tracking of the intracellular transport of clathrin-coated pits and vesicles
作者:
葛云 章东
南京大学电子科学与工程系,南京 210093
Author(s):
Ge Yun Zhang Dong
Department of Electronic Science and Engineering, Nanjing University, Nanjing 210093, China
关键词:
小波分解 显微镜图像 图像分割 目标跟踪 分裂合并
Keywords:
wavelet transform decomposition microscopy image image segmentation target tracking merging and splitting
分类号:
TP391.41
DOI:
10.3969/j.issn.1001-0505.2009.03.009
摘要:
采用基于小波分解多尺度系数乘积的方法检测图像中的斑点,同时提出了基于贪婪思想的可以自动处理目标分裂和合并的模型,综合目标信息对轨迹进行跟踪.对Adaptor和Clathrin图像分割取得了比较理想的结果,Adaptor图像中的目标识别率98.61%,Clathrin图像中目标识别率达到97.65%.新的跟踪算法在处理轨迹合并和分裂方面有明显优势,对斑点跟踪效率达到为98.05%.结果表明,新的算法能有效地识别和跟踪图像中的斑点.从生物学角度,更有效的识别有助于更好地理解质膜的通道运输机制以及因为其不正常工作引起的疾病.
Abstract:
The problem of particle detection and tracking is addressed to understand the processes at the cellular level in live cell imaging. A new tracking approach is proposed based on a greedy algorithm, stable match for point tracking and á-trous wavelet transform decomposition. It can handle track creation, occlusion and termination effectively. The detection rates are 98.61% and 97.65% for Adaptor and Clathrin in microscopy images respectively. The proposed tracking approach demonstrates an obvious advantage in solving merging and splitting issues compared with traditional methods in simulated images, and the tracking accuracy achieves 98.05%. The preliminary results show that the proposed methods are able to identify and track most of the particles successfully, and better understanding can be provided about the Clathrin-dependent pathway of membrane vesicular traffic and the disease ramifications caused by their malfunctions from a biological point of view.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介: 葛云(1970—),男,博士,副教授,geyun@nju.edu.cn.
基金项目: 江苏省教育厅产业化资助项目(JHB06-01).
引文格式: 葛云,章东.活体细胞图像斑点的自动提取和跟踪方法[J].东南大学学报:自然科学版,2009,39(3):464-467. [doi:10.3969/j.issn.1001-0505.2009.03.009]
更新日期/Last Update: 2009-05-20