[1]赵依,吕晓迎,王志功,等.硅表面改性及其生物学研究[J].东南大学学报(自然科学版),2009,39(3):473-476.[doi:10.3969/j.issn.1001-0505.2009.03.011]
 Zhao Yi,Lü Xiaoying,Wang Zhigong,et al.Surface modification of silicon and related biological research[J].Journal of Southeast University (Natural Science Edition),2009,39(3):473-476.[doi:10.3969/j.issn.1001-0505.2009.03.011]
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硅表面改性及其生物学研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
39
期数:
2009年第3期
页码:
473-476
栏目:
生物医学工程
出版日期:
2009-05-20

文章信息/Info

Title:
Surface modification of silicon and related biological research
作者:
赵依1 吕晓迎1 王志功2 姜正林3 李霞3 黄炎1
1 东南大学生物电子学国家重点实验室,南京 210096; 2 东南大学射频与光电集成电路研究所,南京 210096; 3 南通大学江苏省神经再生重点实验室,南通 226001
Author(s):
Zhao Yi1 Lü Xiaoying1 Wang Zhigong2 Jiang Zhenglin3 Li Xia3 Huang Yan1
1 State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
2 Institute of RF and OE-ICs, Southeast University, Nanjing 210096, China
3 Key Laboratory of Neural Regeneration of Jiangsu Province, Nantong University, Nantong 226001, China
关键词:
表面改性 海马神经细胞
Keywords:
silicon surface modification hippocampal neurons
分类号:
R318.08
DOI:
10.3969/j.issn.1001-0505.2009.03.011
摘要:
为了增加海马神经元在以硅为基底的微电子器件表面的黏附和生长,采用化学方法对集成电路的衬底材料硅进行了表面改性,并对改性前后的硅表面进行了X射线光电子能谱分析、原子力显微镜形貌分析和接触角测定.用改良的考马斯亮蓝法对硅片和改性后的硅片进行了蛋白质吸附研究,并采用荧光显微镜观察了胎鼠海马神经细胞在改性前后硅表面的黏附行为.结果表明,改性后的硅表面接触角减小,粗糙度增加.蛋白质吸附结果表明硅的改性能减少蛋白质的吸附.海马神经细胞在改性硅前后表面的黏附行为研究表明,未改性的硅片上几乎不能黏附海马神经细胞,而改性后硅片上能显著增加海马神经细胞的黏附和生长,并能形成神经细胞网络.
Abstract:
In order to improve the adhesion and growth abilities of hippocampal neurons on the surface of silicon-based microelectronic devices, the substrate material of integrated circuit, silicon, was modified by means of a chemical method. The silicon surfaces before and after modification were characterized with X-ray photoelectron spectroscopy(XPS), atomic force microscopy(AFM)and contact angle system. The amount of adsorbed protein on original and modified silicon surfaces was measured by a Coomassie brilliant blue protein assay. Cell adhesion behavior was then assessed by fluorescence microscopy. Results show that the water contact angle becomes smaller and the surface is rougher after modification. Protein adsorption results indicate that modified silicon is efficient in reducing protein adsorption. The experimental results of hippocampal cell cultures demonstrate that a bare silicon wafer makes the neuron adhesion very difficult, but the modified silicon significantly improves neuronal adhesion and growth, and neurons may connect with each other forming a network.

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备注/Memo

备注/Memo:
作者简介: 赵依(1982—),女,硕士生; 吕晓迎(联系人),女,博士,教授,博士生导师,luxy@seu.edu.cn.
基金项目: 国家自然科学基金重点资助项目(90307013)、国家自然科学基金创新群体基金资助项目(60121101).
引文格式: 赵依,吕晓迎,王志功,等.硅表面改性及其生物学研究[J].东南大学学报:自然科学版,2009,39(3):473-476. [doi:10.3969/j.issn.1001-0505.2009.03.011]
更新日期/Last Update: 2009-05-20