[1]向红,吕锡武,尹立红,等.净水工艺中的微生物群落结构与优势菌[J].东南大学学报(自然科学版),2010,40(3):630-635.[doi:10.3969/j.issn.1001-0505.2010.03.037]
 Xiang Hong,Lü Xiwu,Yin Lihong,et al.Microbial community structure and predominant bacteria in water purification process[J].Journal of Southeast University (Natural Science Edition),2010,40(3):630-635.[doi:10.3969/j.issn.1001-0505.2010.03.037]
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净水工艺中的微生物群落结构与优势菌()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
40
期数:
2010年第3期
页码:
630-635
栏目:
环境科学与工程
出版日期:
2010-05-20

文章信息/Info

Title:
Microbial community structure and predominant bacteria in water purification process
作者:
向红123 吕锡武1 尹立红2 朱光灿1 刘冉2 苏耀耀2 杨飞2
1 东南大学能源与环境学院, 南京 210096; 2 东南大学公共卫生学院, 南京 210009; 3 贵州省疾病预防控制中心, 贵阳 550004
Author(s):
Xiang Hong123 Lü Xiwu1 Yin Lihong2 Zhu Guangcan1 Liu Ran2 Su Yaoyao2 Yang Fei2
1 School of Energy and Environment, Southeast University, Nanjing 210096, China
2 School of Public Health, Southeast University, Nanjing 210009, China
3 Guizhou Provincial Center for Disease Prevention and Control, Guiyang 550004, China
关键词:
生物活性炭工艺 生物强化过滤 微生物群落结构 群落多样性 优势菌
Keywords:
biological activated carbon process biologically-enhanced filtration microbial community structure community diversity predominant bacteria
分类号:
X172
DOI:
10.3969/j.issn.1001-0505.2010.03.037
摘要:
采用PCR-单链构象多态性(PCR-SSCP)技术对生物活性炭滤池与生物强化净水工艺中的微生物群落结构和多样性进行分析,并利用最大可能数法研究生物活性炭滤池与生物强化滤池中可培养的优势菌.实验结果表明,混凝、砂滤及生物强化过滤净水工艺对微生物的群落结构均无明显影响; 生物活性炭净水工艺则可使原水微生物群落多样性降低,这是由于活性炭对微生物具有的较强吸附作用所致.通过分析R2A培养基上生长的微生物群落,发现各生物滤池中可培养的微生物群落具有丰富多样性,且滤池间Dice遗传相似系数为0.69~0.86,即群落种类差异较小.生物活性炭滤池与生物强化滤池中可培养的主要优势菌为巨大芽胞杆菌、短小芽胞杆菌、蜡样芽胞杆菌、弗劳地枸橼酸杆菌、豚鼠气单胞菌、恶臭假单胞菌和肺炎克雷氏菌等.将PCR-SSCP技术与传统培养方法相结合,可分析生物滤池中的优势菌群,提高饮用水的生物处理能力.
Abstract:
The microbial community structure and diversity in biological activated carbon and the biologically-enhanced filtration processes are analyzed by polymerase chain reaction single-strand conformation polymorphism(PCR-SSCP). The culturable predominant bacteria in the biological activated carbon filter and the biologically-enhanced active filter are investigated by the most probable number method. The results show that water purification processes of flocculation,sand filtration and biologically-enhanced filtration have no remarkable effect on the microbial community structure. However, due to the powerful adsorption for microorganisms on activated carbon, the diversity of the microbial community of the raw water is reduced after the biological activated carbon process. The analysis results of the cultivable microbial communities on R2A medium show that the cultivable microbial diversity in biofilters is comparatively rich. The Dice similarity coefficients among filters range from 0.69 to 0.86,which indicate that the differences in community species are not obvious. The main culturable predominant bacteria in the biological activated carbon filter and biologically-enhanced active filter are Bacillus megatherium,Bacillus pumilus,Bacillus cereus,Citrobacter freundii,Aeromonas caviae,Pseudomonas putida and Klebsiella pneumoniae. The combination of the PCR-SSCP technique and the conventional culture method can explore predominant bacteria in biofilters and improve efficiency in biological water treatment.

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备注/Memo

备注/Memo:
作者简介: 向红(1965—), 男,博士生; 吕锡武(联系人),男,博士,教授,博士生导师,xiwulu@seu.edu.cn.
基金项目: 江苏省建设厅科技计划资助项目(JS2007-JH22).
引文格式: 向红,吕锡武,尹立红,等.净水工艺中的微生物群落结构与优势菌[J].东南大学学报:自然科学版,2010,40(3):630-635. [doi:10.3969/j.issn.1001-0505.2010.03.037]
更新日期/Last Update: 2010-05-20