[1]丁收年,单丹,张静,等.葡萄糖氧化酶在氧化铋纳米多晶膜中的固定及生物传感[J].东南大学学报(自然科学版),2010,40(6):1327-1331.[doi:10.3969/j.issn.1001-0505.2010.06.038]
 Ding Shounian,Shan Dan,Zhang Jing,et al.Immobilization and biosensing of glucose oxidase in bismuth oxide nanoparticles polycrystalline films[J].Journal of Southeast University (Natural Science Edition),2010,40(6):1327-1331.[doi:10.3969/j.issn.1001-0505.2010.06.038]
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葡萄糖氧化酶在氧化铋纳米多晶膜中的固定及生物传感()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
40
期数:
2010年第6期
页码:
1327-1331
栏目:
化学化工
出版日期:
2010-11-20

文章信息/Info

Title:
Immobilization and biosensing of glucose oxidase in bismuth oxide nanoparticles polycrystalline films
作者:
丁收年1 单丹2 张静2 薛怀国2 孙岳明1
1 东南大学化学化工学院,南京 211189; 2 扬州大学化学化工学院,扬州225002
Author(s):
Ding Shounian1 Shan Dan2 Zhang Jing2 Xue Huaiguo2 Sun Yueming1
1 School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
2 School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
关键词:
葡萄糖传感器 葡萄糖氧化酶 纳米氧化铋
Keywords:
glucose biosensor glucose oxidase bismuth oxide nanoparticles
分类号:
O657.1
DOI:
10.3969/j.issn.1001-0505.2010.06.038
摘要:
以实验室合成的纳米氧化铋(BiOx)为载体,经戊二醛交联,将葡萄糖氧化酶(GOD)固定于铂盘电极表面,从而构筑新型葡萄糖生物传感器.所得修饰电极利用扫描电镜(SEM)、红外光谱(IR)和电化学交流阻抗(EIS)等方法进行了详细表征.扫描电镜显示纳米氧化铋由大量具有二维片状纳米结构和一维纳米线组成; 氧化铋纳米片厚度为80~110 nm,并具有亚微米和微米级别的横向尺寸; 氧化铋纳米棒、线的直径约为40~50 nm.实验结果表明,构筑传感器的最佳条件为:GOD与BiOx的质量比为2:1,酶固载量为30 μg.同时还考察了溶液pH以及操作电位对传感器响应电流的影响.该传感器在0.5 V(vs. SCE)电位下检测葡萄糖,线性范围为1×10-3~1.5 mmol/L,检出限为0.4 μmol/L(信噪比为3),酶催化反应的表观米氏常数为2.88 mmol/L.同时该传感器还表现出响应迅速(5 s)﹑重现性好、使用寿命长等优点.
Abstract:
A novel glucose biosensor was achieved by casting the biocomposite on the surface of platinum disk electrode via the cross-linking step by glutaraldehyde, with the laboratory-prepared bismuth oxide nanoparticles as enzyme immobilization matrix. The modified electrode has been characterized by scanning electron microscopy(SEM), infrared spectroscopy(IR)and electrochemical impedance(EIS)etc. SEM shows that the prepared nano-bismuth oxide is composed by two-dimensional nano-bismuth oxide sheet and one-dimensional nano-rod. The nano-bismuth oxide sheet thickness is about 80 to 110 nm with sub-micron-micron-level horizontal dimensions, and the diameter of bismuth oxide nanorods is about 40 to 50 nm. Experimental results show that the optimal conditions to fabricate biosensor are: GOD and BiOx mass ratio of 2:1, enzyme immobilized amount of 30 μg. The pH and the detection potential effects were also investigated. The biosensor has a wide linear range from 1×10-3 to 1.5 mmol/L with detection limit of 0.4 mmol/L(SNR=3)at 0.5 V vs. SCE. The apparent Michaelis constant is 2.88 mmol/L. And the biosensor presents fast response(5 s), good repeatability and excellent long-term stability.

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备注/Memo

备注/Memo:
作者简介: 丁收年(1975—),男,博士,讲师,snding@seu.edu.cn.
基金项目:国家自然科学基金资助项目(20905011, 20773108)、江苏省自然科学基金资助项目(BK2010396)、东南大学创新基金资助项目(3207040501)、东南大学生物电子学国家重点实验室开放研究基金资助项目.
引文格式: 丁收年,单丹,张静,等.葡萄糖氧化酶在氧化铋纳米多晶膜中的固定及生物传感[J].东南大学学报:自然科学版,2010,40(6):1327-1331. [doi:10.3969/j.issn.1001-0505.2010.06.038]
更新日期/Last Update: 2010-11-20