[1]彭永臻,薛桂松,苗志加,等.葡萄糖为碳源的EBPR长期运行效果及聚磷菌的富集培养[J].东南大学学报(自然科学版),2013,43(1):136-141.[doi:10.3969/j.issn.1001-0505.2013.01.026]
 Peng Yongzhen,Xue Guisong,Miao Zhijia,et al.Long term effect of glucose as sole carbon source on EBPR and PAOs enrichment[J].Journal of Southeast University (Natural Science Edition),2013,43(1):136-141.[doi:10.3969/j.issn.1001-0505.2013.01.026]
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葡萄糖为碳源的EBPR长期运行效果及聚磷菌的富集培养()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
43
期数:
2013年第1期
页码:
136-141
栏目:
环境科学与工程
出版日期:
2013-01-20

文章信息/Info

Title:
Long term effect of glucose as sole carbon source on EBPR and PAOs enrichment
作者:
彭永臻薛桂松苗志加翁冬晨
北京工业大学北京市水质科学与水环境恢复工程重点实验室, 北京100124
Author(s):
Peng Yongzhen Xue Guisong Miao Zhijia Weng Dongchen
Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, Beijing University of Technology, Beijing 100124, China
关键词:
强化生物除磷 葡萄糖 挥发性脂肪酸 聚磷菌 聚羟基脂肪酸酯
Keywords:
enhanced biological phosphorus removal glucose volatile fatty acid polyphosphate accumulating organism glycogen accumulating organism
分类号:
X703.1
DOI:
10.3969/j.issn.1001-0505.2013.01.026
摘要:
研究了连续运行364 d以葡萄糖为碳源的强化生物除磷(EBPR)系统,比较了3个不同运行阶段典型周期的运行状况,考察了厌氧段聚磷菌(PAOs)对有机底物的贮存转化,运用FISH技术分析了系统菌群结构变化.结果表明:随着运行时间的增加PAOs富集程度增高,第180天后反应器最高厌氧释磷量达到80 mg/L,出水磷浓度小于1 mg/L,以葡萄糖为碳源的EBPR系统可以长期高效稳定运行;与第1和第2阶段相比,第3阶段典型周期效果最佳,其厌氧释磷量达到79 mg/L,PO3-4-P去除率达到97.2%;葡萄糖先被发酵细菌分解为挥发性脂肪酸(VFA),PAOs吸收VFA合成聚羟基脂肪酸酯(PHA);荧光原位杂交技术分析发现,PAOs比例不断升高,第340天时比例为45.5%,说明以葡萄糖为碳源的EBPR系统可以富集高浓度的PAOs.
Abstract:
To study the long term effect of glucose on the performance of enhanced biological phosphorus removal(EBPR)system, a laboratory scale sequencing batch reactor(SBR)fed with glucose was operated for 364 days. The SBR performance of three typical cycles in different periods was compared. The effect of glucose on anaerobic substrate storage and conversion of phosphate-accumulating organisms(PAOs)was discussed, and the quantity of populations of PAOs and glycogen accumulating organism(GAOs)was detected with fluorescent in situ hybridization(FISH). The results indicate that the system shows high and stable phosphorus removal efficiency after 180 days, the highest phosphorus release can reach 80 mg/L and the effluent concentration of PO3-4-P is less than 1 mg/L. Compared with the typical cycles in the 1st and 2nd period, the typical cycle in the 3rd period showed the best performance, the anaerobic phosphorus release reaches 79 mg/L and phosphorus release rate is 97.2%. It is indicated that fermentative bacteria first converts glucose to volatile fatty acids(VFAs)and then PAOs utilize the VFAs to synthesize poly-hydro xyalkanoate(PHA). The proportion of PAOs keeps increasing rapidly and reaches 45.5% at 340 days. The FISH analysis results indicate that it is possible for EBPR using glucose as the sole carbon source to develop a system with high proportions of PAOs.

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备注/Memo

备注/Memo:
作者简介: 彭永臻(1949—),男,博士,教授,博士生导师, pyz@bjut.edu.cn.
基金项目: 高等学校博士学科点专项科研基金(优先发展领域)资助项目(20111103130002)、城市污水高效脱氮除磷新工艺与节能降耗关键技术研发资助项目(D121100000112001).
引文格式: 彭永臻,薛桂松,苗志加,等.葡萄糖为碳源的EBPR长期运行效果及聚磷菌的富集培养[J].东南大学学报:自然科学版,2013,43(1):136-141. [doi:10.3969/j.issn.1001-0505.2013.01.026]
更新日期/Last Update: 2013-01-20