[1]许燕波,钱春香,陆兆文.甘油提高巴氏芽孢杆菌脲酶的热稳定性[J].东南大学学报(自然科学版),2013,43(1):147-151.[doi:10.3969/j.issn.1001-0505.2013.01.028]
 Xu Yanbo,Qian Chunxiang,Lu Zhaowen.Study on enhancing urease thermostability of Bacillus pasteurii by glycerol[J].Journal of Southeast University (Natural Science Edition),2013,43(1):147-151.[doi:10.3969/j.issn.1001-0505.2013.01.028]
点击复制

甘油提高巴氏芽孢杆菌脲酶的热稳定性()
分享到:

《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
43
期数:
2013年第1期
页码:
147-151
栏目:
生物医学工程
出版日期:
2013-01-20

文章信息/Info

Title:
Study on enhancing urease thermostability of Bacillus pasteurii by glycerol
作者:
许燕波钱春香陆兆文
东南大学材料科学与工程学院, 南京211189; 东南大学江苏省土木工程材料重点实验室, 南京211189
Author(s):
Xu Yanbo Qian Chunxiang Lu Zhaowen
School of Materials Science and Engineering Southeast University, Nanjing 211189, China
Jiangsu Key Laboratory of Construction Materials, Southeast University, Nanjing 211189, China
关键词:
碳酸盐矿化菌 脲酶 甘油 热稳定性
Keywords:
carbonate-mineralization microbe urease glycerol thermostability
分类号:
Q89
DOI:
10.3969/j.issn.1001-0505.2013.01.028
摘要:
研究了保存时间和保存温度对碳酸盐矿化菌脲酶活性的影响,发现在低温4 ℃条件下,脲酶活性在29 d内出现不同程度下降;常温短期储存时,酶活性可以保持3 d,3 d后酶活性值下降明显.为提高脲酶的热稳定性,向碳酸盐矿化菌中添加甘油作为保护剂,常温下添加总体积分数20%的甘油,可以提高酶的稳定性,脲酶活性在30 ℃下3 d内保持不变,但甘油保护作用有限,高温储存超过7 d后酶活性值下降明显;而在保存温度4 ℃条件下,添加体积分数为10%的甘油,碳酸盐矿化菌可以储存30 d,底物分解量可以达到初始菌液的95%.
Abstract:
Effect of preservation time and temperature on the carbonate-mineralization microbe urease activity is studied. The urease activity decreases in 29 days at 4 ℃ while it can be kept for 3 days at normal temperature before obvious decrease afterwards. Glycerol is selected as the protective additive to enhance the urease thermostability. The urease activity keep stable for 3 days at 30 ℃, and the best volume fraction for glycerol is 20% at normal temperature. However its effect of preservation is limited, and the urease activity decreases obviously after 7 days at high store temperature. The decomposition ability can reach up to 95% at 4 ℃ after 30 days with 10% glycerol, and the carbonate-mineralization microbe can be kept in reserve for a month.

参考文献/References:

[1] 李沛豪,居文俊. 细菌诱导碳酸钙矿化材料及其应用前景[J]. 建筑材料学报,2009,12(4): 482-486.
  Li Peihao, Ju Wenjun. Applications of calcium carbonate mineralization material induced by bacterium[J]. Journal of Building Materials, 2009,12(4):482-486.(in Chinese)
[2] 黄琰,罗学刚,何晶,等. 微生物在石英砂中诱导方解石沉积的实验研究[J]. 西南科技大学学报, 2009, 24(2):65-69.
  Huang Yan, Luo Xuegang, He Jing, et al. Studies on the experiment of microbiologically-induced calcite precipitation in silica[J]. Journal of Southwest University of Science and Technology,2009, 24(2):65-69.(in Chinese)
[3] 黄琰,罗学刚,杜菲. 微生物诱导方解石沉积加固的影响因素[J]. 西南科技大学学报, 2009,24(3):87-93.
  Huang Yan, Luo Xuegang, Du Fei. Studies on the effect factor of microbiologically-induced calcite precipitation[J]. Journal of Southwest University of Science and Technology, 2009, 24(3):87-93.(in Chinese)
[4] 成亮, 钱春香, 王瑞兴, 等. 碳酸盐矿化菌株固结土壤Cd2+的生物矿化过程[J].硅酸盐学报,2008, 36(增刊1): 216-222.
  Cheng Liang, Qian Chunxiang, Wang Ruixing, et al. Bioremedidation process of Cd2+ removal from soil by bacteria biomineralization[J].Chinese Ceramic Society, 2008,36(S1):216-222.(in Chinese)
[5] Sondi I, Matijevic E. Homogeneous precipitation by enzyme-catalyzed reactions-strontium and barium carbonates[J]. Chemistry of Materials, 2003, 15(6):1322-1326.
[6] 李丽. 微生物酶促形成方解石胶结砂粒的实验与模拟[D]. 南京:东南大学材料科学与工程学院,2011.
[7] 刘朝辉, 武伟娜, 刘跃, 等. 保护剂提高β-甘露聚糖酶热稳定性的研究[J]. 天津大学学报,2008,41(1):114-118.
  Liu Zhaohui, Wu Weina, Liu Yue, et al. Enhancing the thermostability of β-mannanase by protective additives[J]. Journal of Tianjin University, 2008, 41(1):114-118.(in Chinese)
[8] 郑贤良, 吴丹, 李兆丰, 等. 化学添加剂提高重组α-环糊精葡萄糖基转移酶酶制剂稳定性[J]. 生物工程学报,2011,27(2):185-195.
  Zheng Xianliang, Wu Dan, Li Zhaofeng, et al. Enhanced storage stability of recombinant enzyme preparation of α-CGTase from Paenibacillus macerans by chemical additives[J]. Chinese Journal of Biotechnology, 2011,27(2):185-195.(in Chinese)
[9] 李亚星,徐秋明,曹一平,等. 分光光度法测定树脂包衣尿素溶出的研究[J]. 中国土壤与肥料,2010(1):84-87.
  Li Yaxing, Xu Qiuming, Cao Yiping, et al. Colorimetric estimation of urea release rate in coated urea[J]. Soil and Fertilizer Science in China, 2010(1):84-87.(in Chinese)

相似文献/References:

[1]王国芳,吴磊,徐雪玲,等.蚯蚓对垂直流人工湿地微生物量及脲酶活性的影响[J].东南大学学报(自然科学版),2010,40(5):1013.[doi:10.3969/j.issn.1001-0505.2010.05.025]
 Wang Guofang,Wu Lei,Xu Xueling,et al.Influence of Eisenia foetida on biomass and urease activity in vertical constructed wetland[J].Journal of Southeast University (Natural Science Edition),2010,40(1):1013.[doi:10.3969/j.issn.1001-0505.2010.05.025]
[2]黄娟,王世和,鄢璐,等.潜流型人工湿地的脲酶活性分布特性[J].东南大学学报(自然科学版),2008,38(1):166.[doi:10.3969/j.issn.1001-0505.2008.01.032]
 Huang Juan,Wang Shihe,Yan Lu,et al.Distribution characteristic of urease activity in subsurface constructed wetlands[J].Journal of Southeast University (Natural Science Edition),2008,38(1):166.[doi:10.3969/j.issn.1001-0505.2008.01.032]

备注/Memo

备注/Memo:
作者简介: 许燕波(1986—),男,硕士生;钱春香(联系人),女,博士,教授,博士生导师, cxqian@seu.edu.cn.
基金项目: 江苏省科技支撑计划资助项目(BK2010062).
引文格式: 许燕波,钱春香,陆兆文.甘油提高巴氏芽孢杆菌脲酶的热稳定性[J].东南大学学报:自然科学版,2013,43(1):147-151. [doi:10.3969/j.issn.1001-0505.2013.01.028]
更新日期/Last Update: 2013-01-20