[1]杨小丽,周娜,陈明,等.FISH技术解析不同氨氮浓度MBR中的微生物群落结构[J].东南大学学报(自然科学版),2013,43(2):380-385.[doi:10.3969/j.issn.1001-0505.2013.02.029]
 Yang Xiaoli,Zhou Na,Chen Ming,et al.Analysis of microbial community structure in MBR with different ammonia concentrations using fluorescence in situ hybridization[J].Journal of Southeast University (Natural Science Edition),2013,43(2):380-385.[doi:10.3969/j.issn.1001-0505.2013.02.029]
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FISH技术解析不同氨氮浓度MBR中的微生物群落结构()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
43
期数:
2013年第2期
页码:
380-385
栏目:
环境科学与工程
出版日期:
2013-03-20

文章信息/Info

Title:
Analysis of microbial community structure in MBR with different ammonia concentrations using fluorescence in situ hybridization
作者:
杨小丽1周娜1陈明1张瑞1宋海亮2傅大放1
1东南大学土木工程学院, 南京 210096; 2东南大学能源与环境学院, 南京 210096
Author(s):
Yang Xiaoli1 Zhou Na1 Chen Ming1 Zhang Rui1 Song Hailiang2 Fu Dafang1
1School of Civil Engineering, Southeast University, Nanjing 210096, China
2School of Energy and Environment, Southeast University, Nanjing 210096, China
关键词:
膜生物反应器 荧光原位杂交 微生物群落结构 变形菌 氨氧化细菌 硝化细菌
Keywords:
membrane bioreactor fluorescence in situ hybridization microbial community structure Proteobacteria Ammonia-oxidizing bacteria Nitrobacter sp.
分类号:
X703.1
DOI:
10.3969/j.issn.1001-0505.2013.02.029
摘要:
为了探究膜生物反应器(MBR)中微生物群落结构与硝化作用的内在关系,采用荧光原位杂交技术(FISH)对不同氨氮浓度的MBR系统中微生物种群进行检测,考察变形菌(Proteobacteria)、放线菌(Actinobacteria)、黄杆菌(Cytophaga-flexibacter-bacteroides)、绿弯菌(Chloroflexi)、硝化细菌(Nitrobacter sp.)和氨氧化细菌(Ammonia-oxidizing bacteria)占总细菌的相对含量.结果表明:进水氨氮浓度分别为35,3.47和100 mg/L的MBR系统中,变形菌分别占总细菌的51%,44%和39%,氨氧化细菌与硝化细菌之和分别占总细菌的28%,4%和43%.在氨氮浓度较低的MBR中,检测到大量丝状β-变形菌,同时放线菌和绿弯菌也有所增加.高氨氮负荷有利于氨氧化细菌和硝化细菌的生长富集,并使其在系统中成为优势种群.相关性分析表明,氨氧化细菌、硝化细菌与氨氮去除呈显著正相关.
Abstract:
The intrinsic relationship between the microbial community structure and nitrification was investigated in the membrane bioreactor(MBR)with different influent NH+4-N concentrations. Fluorescence in situ hybridization(FISH)was adopted for analyzing microbial community structure. Proteobacteria, Actinobacteria, Cytophaga-flexibacter-bacteroides, Chloroflexi, Nitrobacter sp. and Ammonia-oxidizing bacteria were detected. The results show that when the influent NH+4-N concentrations are 35, 3.47 and 100 mg/L, Proteobacteria accounts for 51%, 44% and 39% of total bacteria, while the sum of Ammonia-oxidizing and Nitrobacter sp. accounts for 28%, 4% and 43%, respectively. Abundant filamentous Betaproteobacteria is detected in MBR with lower NH+4-N concentration. Actinobacteria and Chloroflexi are also found to be increased simultaneously. Ammonia-oxidizing bacteria and Nitrobacter sp. are enriched and dominant in MBR with higher NH+4-N load. Correlation analysis indicates that Ammonia-oxidizing bacteria and Nitrobacter sp. are positively correlated with NH+4-N removal.

参考文献/References:

[1] Judd S J. The status of membrane bioreactor technology[J]. Trends in Biotechnology, 2008, 26(2): 109-116.
[2] Amann R, Fuchs B M. Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques[J]. Nature Reviews Microbiology, 2008, 6(5): 339-348.
[3] Snaidr J, Amann R, Huber I, et al. Phylogenetic analysis and in situ identification of bacteria in activated sludge[J]. Applied and Environmengtal Microbiology, 1997, 59(1): 143-169.
[4] 朱琳,尹立红,浦跃朴,等. 荧光原位杂交法检测环境硝化细菌实验条件优化及应用[J].东南大学学报:自然科学版, 2005, 35(2): 266-270.
  Zhu Lin, Yin Lihong, Pu Yuepu, et al. Optimization and application of fluorescence in situ hybridization assay for detecting nitrifying bacteria in environmental samples[J]. Journal of Southeast University: Natural Science Edition, 2005, 35(2): 266-270.(in Chinese)
[5] Britt-Marie W, Motoharu O, Malte H, et al. Microbial community structure in activated sludge floc analysed by fluorescence in situ hybridization and its relation to floc stability [J]. Water Research, 2008, 42(8): 2300-2308.
[6] He Shuying, Chen Jing, Li Jixiang. Dynamics of Nitrobacteria community in biological contact oxidation process by DGGE and FISH[C]//4th International Conference on Bioinformatics and Biomedical Engineering. Chengdu, China, 2010: 5515470.
[7] Lubarsky H V, Hubas C, Chocholek M, et al. The stabilisation potential of individual and mixed assemblages of natural bacteria and microalgae[J]. PLoS one, 2010, 5(11): 1-12.
[8] Manz W, Amann R, Ludwig W, et al. Application of a suite of 16S rRNA specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacter-Bacteroides in the natural environment[J]. Microbiology,1996, 142(5): 1097-1106.
[9] Björnsson L, Hugenholtz P, Tyson G W, et al. Filamentous Chloroflexi(green non-sulfur bacteria)are abundant in wastewater treatment processes with biological nutrient removal[J]. Microbiology, 2002, 148(8): 2309-2318.
[10] Roller C, Wagner M, Amann R, et al. In situ probing of Gram-positive bacteria with high G+C content using 23S rRNA-targeted oligonucleotides[J]. Microbiology, 1994, 140(10): 2849-2858.
[11] 杨宗政,顾平,刘静文.好氧序批式MBR处理高浓氨氮废水[J].中国给水排水,2005,21(3):53-56.
  Yang Zongzheng, Gu Ping, Liu Jingwen. Aerobic sequencing MBR for treatment high concentration of ammonia nitrogen wastewater[J]. China Water & Wastewater, 2005, 21(3): 53-56.(in Chinese)
[12] Zang K, Kurisu F, Kasuga I, et al. Analysis of the phylogenetic diversity of estrone-degrading bacteria in activated sewage sludge using microautoradiography-fluorescence in situ hybridization[J]. Systematic and Applied Microbiology, 2008, 31(3): 206-214.
[13] Mota C, Head M A, Ridenoure J A, et al. Effects of aeration cycles on nitrifying bacterial populations and nitrogen removal in intermittently aerated reactors[J]. Applied and Environmental Microbiology, 2005, 71(12): 8565-8572.
[14] 张斌,孙宝盛,刘慧娜,等. 处理不同废水MBR系统中微生物群落结构的比较[J].环境科学, 2008,29(10): 2943-2949.
  Zhang Bin, Sun Baosheng, Liu Huina, et al. Comparison of microbial community structure in MBRs treating different wastewater[J]. Environmental Science, 2008, 29(10): 2943-2949.(in Chinese)
[15] Kragelund C, Kong Y, Thelen K, et al. Ecophysiology of different filamentous Alphaproteobacteria species from industrial waste water treatment plants[J]. Microbiology, 2006, 152(10): 3003-3012.
[16] 马迪根M T, 马丁克J M.BROCK微生物生物学:上册[M].11版.李春明,杨文博,译.北京:科学出版社,2009:592-593.
[17] 高大文,李昕芯,安瑞,等.不同DO下MBR内微生物群落结构与运行效果关系[J].中国环境科学,2010,30(2):209-215.
  Gao Dawen, Li Xinxin, An Rui,et al. Relationships between microbial community structure and the performance of MBR under different dissolved oxygen[J]. China Environmental Science, 2010, 30(2): 209-215.(in Chinese)

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备注/Memo

备注/Memo:
作者简介: 杨小丽(1977—),女,博士,副教授,yangxiaoli@seu.edu.cn.
基金项目: 国家自然科学基金资助项目(51008064,51109038)、江苏省高校优势学科建设工程资助项目(1105007001)、东南大学重大科学研究引导基金资助项目(3205002107).
引文格式: 杨小丽,周娜,陈明,等.FISH技术解析不同氨氮浓度MBR中的微生物群落结构[J].东南大学学报:自然科学版,2013,43(2):380-385. [doi:10.3969/j.issn.1001-0505.2013.02.029]
更新日期/Last Update: 2013-03-20