[1]常旭红,陈璐斯,杨天,等.气管滴注纳米二氧化钛对大鼠的亚急性肺毒性实验研究[J].东南大学学报(自然科学版),2014,44(3):616-620.[doi:10.3969/j.issn.1001-0505.2014.03.029]
 Chang Xuhong,Chen Lusi,Yang Tian,et al.Experimental study on subacute pulmonary toxicity induced by intra-tracheal instillation of nano-TiO2 in rats[J].Journal of Southeast University (Natural Science Edition),2014,44(3):616-620.[doi:10.3969/j.issn.1001-0505.2014.03.029]
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气管滴注纳米二氧化钛对大鼠的亚急性肺毒性实验研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
44
期数:
2014年第3期
页码:
616-620
栏目:
生物医学工程
出版日期:
2014-05-16

文章信息/Info

Title:
Experimental study on subacute pulmonary toxicity induced by intra-tracheal instillation of nano-TiO2 in rats
作者:
常旭红12陈璐斯12杨天12唐萌13王蓓12
1东南大学环境医学工程教育部重点实验室, 南京210009; 2东南大学公共卫生学院, 南京210009; 3东南大学江苏省生物材料与器件重点实验室, 南京210009
Author(s):
Chang Xuhong12 Chen Lusi12 Yang Tian12 Tang Meng13 Wang Bei12
1Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Southeast University, Nanjing 210009, China
2School of Public Health, Southeast University, Nanjing 210009, China
3Jiangsu Key Lab
关键词:
纳米二氧化钛 肺毒性 氧化应激 亚急性毒性
Keywords:
nano-TiO2 pulmonary injury oxidative stress subacute toxicity
分类号:
R994.6;R318
DOI:
10.3969/j.issn.1001-0505.2014.03.029
摘要:
雄性SD大鼠气管滴注不同剂量的纳米二氧化钛(TiO2),每周2次,连续4周,染毒结束后,检测大鼠血常规、肺组织氧化应激指标和CRP表达,观察肺组织病理学和超微结构改变.结果表明:纳米TiO2 32 mg/kg组血常规中NEUT计数增加,纳米TiO2引起肺组织中SOD,GSH和CAT含量降低,组织病理学显示随着纳米TiO2染毒剂量增加,肺泡扩张不良、闭塞比例增加,肺内吞噬棕黄色颗粒的巨噬细胞数量增加,肺泡间隔内淋巴细胞和纤维细胞增多;超微结构发现纳米TiO2主要引起Ⅱ型肺泡结构改变.结果显示,在本实验条件下纳米TiO2使大鼠的肺组织受到氧自由基的攻击,肺脏清除氧自由基的能力减弱,并引起肺组织不同程度的病理和超微结构的改变.
Abstract:
Sprague Dawley rats were exposed to intra-tracheal instillation of nano-TiO2 with different doses. The exposure was conducted twice a week, for four consecutive weeks. After four weeks, the subacute pulmonary toxicity was assessed by the hematological parameters, enzyme activities, C-reactive protein(CRP)level, pathological examination and transmission electron microscopy(TEM). Results show that the neutrophil count(NEUT)increased in the group of 32 mg/kg nano-TiO2 and the content of hydrogen peroxide(CAT), glutathione(GSH)and superoxide dismutase(SOD)activities decreased. The results of lung histology demonstrate the expansion of lung gaps and slight alveolar thickness, increased proliferation of tissue macrophages of deposition massive particulate, increased lymphocytes and fibrocyte with the increase of nano TiO2 dose. Type II cells have prominent lamellar bodies and the damage of lamellar bodies are observed by TEM. Nano-TiO2can induce the change of enzyme activities and histology, and present inflammation and massive particulate deposition in pulmonary tissues and pulmonary injury.

参考文献/References:

[1] Pietroiusti A. Health implication of engineered nanomaterials[J]. Nanoscale, 2012, 4(4): 1231-1247.
[2] Kateb B, Chiu K, Black K L, et al. Nanoplatforms for constructing new approaches to cancer treatment, imaging, and drug delivery: what should be the policy?[J]. Neuroimage, 2011, 54(1): 106-124.
[3] Sager T M, Castranova V. Surface area of particle administered versus mass in determining the pulmonary toxicity of ultrafine and fine carbon black: comparison to ultrafine titanium dioxide[J]. Particle and Fibre Toxicology, 2009, 4(6): 1-12.
[4] Warheit D B, Thomas R, Kenneth L R, et al. Pulmonary toxicity study in rats with three forms of ultrafine-TiO2 particles: differential responses related to surface properties[J]. Toxicology, 2007, 230(1): 90-104.
[5] Tsai C J, Huang C Y, Chen S C, et al. Exposure assessment of nano-sized and respirable particles at different workplaces[J]. Journal of Nanoparticle Research, 2011, 13(9): 4161-4172.
[6] Iavicoli I, Leso V, Bergamaschi A. Toxicological effects of titanium dioxide nanoparticles: a review of in vivo studies[J]. Journal of Nanomaterials, 2012, 15(5): 481-508.
[7] Johnston H J, Hutchison G R, Christensen F M, et al. Identification of the mechanisms that drive the toxicity of TiO2 particulates: the contribution of physicochemical characteristics[J]. Particle and Fibre Toxicology, 2009, 6(33): 1-27.
[8] Ma L L, Zhao J F, Wang J, et al. The acute liver injury in mice caused by nano-anatase TiO2[J]. Nanoscale Research Letters, 2009, 4(11): 1275-1285.
[9] Tang M, Zhang T, Xue Y Y, et al. Metabonomic studies of biochemical changes in the serum of rats by intratracheally instilled TiO2 nanoparticles[J]. Journal of Nanoscience and Nanotechnology, 2011, 11(4): 3065-3074.
[10] Duan Y M, Liu J, Ma L, et al. Toxicological characteristics of nanoparticulate anatase titanium dioxide in mice[J]. Biomaterials, 2010, 31(5): 894-899.
[11] Xue Y Y, Zhang S S, Huang Y M, et al. Acute toxic effects and gender-related biokinetics of silver nanoparticles following an intravenous injection in mice[J]. Journal of Applied Toxicology, 2012, 32(11): 890-899.
[12] 刘青, 薛秀玲, 叶静, 等. 纳米二氧化钛对小鼠肺、脑和肝脏组织的影响[J].华侨大学学报:自然科学版, 2009, 30(2): 179-184.
  Liu Qing, Xue Xiuling, Ye Jing, et al. The effects of liver, brain and kidney induced by nano TiO2 in mouse [J]. Journal of Huaqiao University:Natural Science, 2009, 30(2): 179-184.(in Chinese)
[13] 张婷, 唐萌, 王姝, 等. 大鼠肺灌注纳米二氧化钛颗粒对其脏器氧化损伤的影响[J].南开大学学报:自然科学版, 2008, 41(3): 24-27.
  Zhang Ting, Tang Meng, Wang Shu, et al. The effects of oxidative stress induced by tracheal instillation nano TiO2 in rats [J]. Acta Scientiarum Naturalium Universitatis Nankaiensis, 2008,41(3): 24-27.(in Chinese)
[14] Ruckerl R, Greven S, Ljungman P, et al. Air pollution and inflammation(interle-ukin-6, c-reactive protein, fibrinogen)in myocardial infarction survivors[J]. Environmental Health Perspectives, 2007, 115(7): 1072-1080.
[15] Lu X Y, Li Y S, Milomir O, et al. Decay-accelerating factor attenuates c-reactive protein-potentiated tissue injury after mesenteric ischemia/reperfusion[J]. Journal of Surgical Research, 2011, 167(2): 103-115.
[16] 曹敬银, 陆元培. C反应蛋白在呼吸系统疾病中的研究进展[J].临床肺科杂志, 2010, 15(9): 1290-1291.
  Cao Jingyin, Lu Yuanpei. The research progress of C-reactive protein in respiratory disease[J]. Journal of Clinical Pulmonary Medicine, 2010, 15(9): 1290-1291.(in Chinese)
[17] Yue W, Schneider A, Stozel M, et al. Ambient source-specific particles are associated with prolonged repolarization and increased levels of inflammation in male coronary artery disease patients[J]. Mutation Research—Fundamental and Molecular Mechanisms of Mutagenesis, 2007, 621(1): 50-60.

备注/Memo

备注/Memo:
收稿日期: 2013-10-16.
作者简介: 常旭红(1982—),男,博士生; 王蓓(联系人),女,博士,教授,博士生导师,wangbeilxb@gmail.com.
基金项目: 国家重大科学研究计划资助项目(2011CB933404)、中央高校基本科研业务费专项资金资助项目(CXZZ12-0122).
引用本文: 常旭红,陈璐斯,杨天,等.气管滴注纳米二氧化钛对大鼠的亚急性肺毒性实验研究[J].东南大学学报:自然科学版,2014,44(3):616-620. [doi:10.3969/j.issn.1001-0505.2014.03.029]
更新日期/Last Update: 2014-05-20