[1]刘芳,赵鑫,潘玉瑾,等.高效异养硝化细菌富集与强化脱氮[J].东南大学学报(自然科学版),2016,46(4):807-811.[doi:10.3969/j.issn.1001-0505.2016.04.022]
　Liu Fang,Zhao Xin,Pan Yujin,et al.Enrichment and enhanced nitrogen removal of heterotrophic nitrifying bacteria[J].Journal of Southeast University (Natural Science Edition),2016,46(4):807-811.[doi:10.3969/j.issn.1001-0505.2016.04.022]
点击复制

高效异养硝化细菌富集与强化脱氮()
分享到：

《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
46
期数:
2016年第4期
页码:
807-811
栏目:
环境科学与工程
出版日期:
2016-07-20

文章信息/Info

Title:
Enrichment and enhanced nitrogen removal of heterotrophic nitrifying bacteria
作者:
刘芳; 赵鑫; 潘玉瑾; 郭惠宣; 林琦; 胡筱敏
东北大学资源与土木工程学院, 沈阳 110819
Author(s):
Liu Fang;  Zhao Xin;  Pan Yujin;  Guo Huixuan;  Lin Qi;  Hu Xiaomin
School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China
关键词:
高效异养硝化菌;  高通量测序;  生物强化;  脱氮;  SBR
Keywords:
heterotrophic nitrifying bacteria;  high-throughput sequencing;  bioaugmentation;  nitrogen removal;  sequencing batch reactor
分类号:
X52
DOI:
10.3969/j.issn.1001-0505.2016.04.022
摘要:
以污水处理厂生化池活性污泥作为菌源,在SBR反应装置中进行异养硝化菌富集.富集后,微生物在pH值为7.5,DO为3~4 mg/L和温度为35 ℃时,NH+4-N去除效率达到99%,高通量测序显示异养硝化细菌Arthrobacter, Bacillus和Pseudomonas之和占65.35%;以其作为接种物,投加到SBR中进行生物强化实验.结果表明,投加异养硝化菌群不仅提高了系统的硝化效率,还可以缩短水力停留时间;在进水NH+4-N质量浓度提高到100 mg/L时,出水NH+4-N质量浓度仍稳定在5 mg/L以下,NH+4-N去除效率远高于对照组.
Abstract:
The enrichment of heterotrophic nitrifying bacteria was carried out in a SBR(sequencing batch reactor)with activated sludge in the biochemical pool as inoculum. The NH+4-N removal rate reached 99% under the conditions that pH was 7.5, DO was 3 to 4 mg/L, and temperature was 35 ℃ after the enrichment. The high-throughput sequencing results show that the main heterotrophic nitrifying bacteria are Arthrobacter, Bacillus, and Pseudomonas, and the sum of these three species is 65.35%. Heterotrophic nitrifying bacteria were added to SBR to treat simulated sewage. The results show that the nitrification efficiency is improved and the HRT(hydraulic retention time)is shortened after being inoculated nitrifying bacteria. When the influent NH+4-N concentration is improved to 100 mg/L, the effluent NH+4-N concentration of the experiment group is under 5 mg/L, and thus it is much less than the control group.

参考文献/References:

[1] 孙锦宜. 含氮废水处理技术与应用[M]. 北京: 化学工业出版社, 2004:33.
[2] Peng Y Z, Zhu G B. Biological nitrogen removal with nitrificaition and denitrification via nitrite pathway[J]. Appl Microbiol Biotechnol, 2006,73:15-26.
[3] 濮培民, 李正魁, 王国祥. 提高水体净化能力控制湖泊富营养化[J]. 生态学报, 2005, 25(10): 2757-2763. DOI:10.3321/j.issn:1000-0933.2005.10.043.
　　Pu Peimin, Li Zhengkui, Wang Guoxiang. Controlling eutrophication by enhancing purification capacity in lakes[J]. Acta Ecologica Sinica, 2005, 25(10):2757-2763. DOI:10.3321/j.issn:1000-0933.2005.10.043.(in Chinese)
[4] 温东辉, 唐孝炎. 异养硝化及其在污水脱氮中的作用[J]. 环境污染与防治, 2003, 25(5): 283-285. DOI:10.3969/j.issn.1001-3865.2003.05.011.
　　Wen Donghui, Tang Xiaoyan. Heterotrophic nitrification and its role in the nitrogen removal in wastewater treatment[J]. Environmental Pollution and Control, 2003, 25(5):283-285. DOI:10.3969/j.issn.1001-3865.2003.05.011.(in Chinese)
[5] 陈赵芳, 尹立红, 浦跃朴, 等. 一株异养硝化菌的筛选及其脱氮条件[J]. 东南大学学报(自然科学版), 2007, 37(3):486-490. DOI:10.3321/j.issn:1001-0505.2007.03.026.
　　Chen Zhaofang, Yin Lihong, Pu Yuepu, et al. Screening of a heterotrophic nitrifying bacterium strain and its optimal conditions for nitrogen removing[J]. Journal of Southeast University(Natural Science Edition), 2007, 37(3):486-490. DOI:10.3321/j.issn:1001-0505.2007.03.026.(in Chinese)
[6] 林燕, 孔海南, 何义亮, 等. 异养硝化细菌的分离及其脱氮特性实验研究[J]. 环境科学, 2006, 27(2): 324-328.
　　Lin Yan, Kong Hainan, He Yiliang, et al. Isolation and characterization of heterotrophic nitrifying bacteria[J]. Environmental Science, 2006, 27(2): 324-328.(in Chinese)
[7] 孔庆鑫, 李君文, 王新为, 等. 一种新的好氧反硝化菌筛选方法的建立及新菌株的发现[J]. 应用与环境生物学报, 2005, 11(2): 222-225.
　　Kong Qingxing, Li Junwen, Wang Xinwei, et al. A new screening method for aerobic denitrification bacteria and isolation of a novel strain[J]. Chinese Journal of Applied and Environmental Biology, 2005, 11(2): 222-225.(in Chinese)
[8] Kim J K, Park K J, Cho K S, et al. Aerobic nitrification-denitrification by heterotrophic Bacillus strains[J]. Bioresource Technology, 2005, 96(17):1897-1906. DOI:10.1016/j.biortech.2005.01.040.
[9] Kundu P, Pramanik A, Mitra S, et al. Heterotrophic nitrification by Achromobacter xylosoxidans S18 isolated from a small-scale slaughterhouse wastewater[J]. Bioprocess and Biosystems Engineering, 2012, 35(5):721-728. DOI:10.1007/s00449-011-0652-6.
[10] 蒋展鹏.环境工程学[M]. 北京: 高等教育出版社, 2005: 19.
[11] Levantesi C, Beimfohr C, Geurkink B, et al. Filamentous aplhaproteobacteria associated with bulking in industrial wastewater treatment plants[J]. Systematic and Applied Microbiology, 2004, 27(6): 716-727. DOI:10.1078/0723202042369974.
[12] Kaetzke A, Jentzsch D, Eschrich K. Quantification of microthrix pravicella in activated sludge bacterial communities by real-time PCR[J]. Letters in Applied Microbiology, 2005, 40(3): 207-211. DOI:10.1111/j.1472-765X.2005.01656.x.
[13] Kumari S K S, Marrengane Z, Bux F. Application of quantitative RT-PCR to determine the distribution of Microthrix parivcella in full-scale activated sludge treatmen systems[J]. Applied Microbiology and Biotechnology, 2009,83(6): 1135-1141. DOI:10.1007/s00253-009-2013-9.
[14] Ho K L,Chung Y C, Tseng C P. Continuous deodorization and bacterial community analysis of a biofilter treating nitrogen-containing gases from swine waste storage pits[J]. Bioresource Technology, 2008, 99(8):2757-2765. DOI:10.1016/j.biortech.2007.06.041.
[15] Verstraete W, Alexander M. Heterotrophic nitrification by Arthrobacter sp.[J]. Journal of Bacteriology, 1972, 110(3): 955-961.
[16] 窦赫喆,侯超,高鹏,等. 高效净水异养硝化细菌的筛选鉴定及固定化应用研究[J]. 工业微生物, 2014, 44(6): 49-53. DOI:10.3969/j.issn.1001-6678.2014.06.009.
　　Dou Hezhe, Hou Chao, Gao Peng, et al. Screening and identification of heterotrophic nitrifier for efficiently treating aquaculture water and its immobilization[J]. Industrial Microbiology, 2014, 44(6):49-53. DOI:10.3969/j.issn.1001-6678.2014.06.009.(in Chinese)
[17] Yang X P, Wang S M, Zhang D W, et al. Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying-denitrifying bacterium, Bacillus subtilis A1[J]. Bioresource Technology, 2011, 102(2): 854-862. DOI:10.1016/j.biortech.2010.09.007.
[18] 尹明锐, 汪苹, 刘健楠, 等. 具有N2O控逸能力的异养硝化-好氧反硝化菌株的筛选鉴定[J]. 环境科学研究, 2010, 23(4): 515-520.
　　Yin Mingrui, Wang Ping, Liu Jiannan, et al. Screening and identification of a heterotrophic nitrification-aerobic denitrification strain with N2O emission control ability[J]. Research of Environmental Sciences, 2010,23(4): 515-520.(in Chinese)
[19] Daum M, Zimmer W, Papen H, et al. Physiological and molecular biological characterization of ammonia oxidation of the heterotrophic nitrifier pseudomonas putida [J]. Current Microbiology, 1998, 37(4):281-288.DOI:10.1007/s002849900379.
[20] 张峰峰, 谢凤型, 周可,等. 一株高氨氮去除能力菌株的分离筛选及鉴定[J]. 华北农学报, 2014, 29(4): 221-225.
　　Zhang Fengfeng, Xie Fengxing, Zhou Ke, et al. Isolation and identification of one bacterium species with high effective ability of removing ammonia-nitrogen[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(4):221-225.(in Chinese)
[21] 刘晶晶, 汪苹, 王欢. 一株异养硝化-好氧反硝化菌的脱氮性能研究[J]. 环境科学研究, 2008, 21(3): 121-125.
　　Liu Jingjing, Wang Ping, Wang Huan. Study on denitrification characteristics of a heterotrophic nitrification-aerobic denitrifier[J]. Research of Environmental Sciences, 2008, 21(3):121-125.(in Chinese)
[22] 梁麟. 异养硝化菌强化好氧颗粒污泥脱氮实验研究[D]. 兰州:兰州理工大学土木工程学院, 2011.

备注/Memo

备注/Memo:
收稿日期: 2015-12-01.
作者简介: 刘芳(1988—),女,博士生;胡筱敏(联系人),男,教授,博士生导师,hxmin_jj@163.com.
基金项目: 国家水体污染控制与治理重大专项资助项目(2013ZX07202-010).
引用本文: 刘芳,赵鑫,潘玉瑾,等.高效异养硝化细菌富集与强化脱氮[J].东南大学学报(自然科学版),2016,46(4):807-811. DOI:10.3969/j.issn.1001-0505.2016.04.022.
更新日期/Last Update: 2016-07-20