[1]陈盛火,张权,Mwanza Christopher,等.唾液及头发中内源性大麻素的高效液相色谱-串联质谱检测[J].东南大学学报(自然科学版),2017,47(3):534-538.[doi:10.3969/j.issn.1001-0505.2017.03.020]
 Chen Shenghuo,Zhang Quan,Mwanza Christopher,et al.Detection of endocannabinoids in saliva and hair by high performance liquid chromatography tandem mass spectrometry[J].Journal of Southeast University (Natural Science Edition),2017,47(3):534-538.[doi:10.3969/j.issn.1001-0505.2017.03.020]
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唾液及头发中内源性大麻素的高效液相色谱-串联质谱检测()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
47
期数:
2017年第3期
页码:
534-538
栏目:
化学化工
出版日期:
2017-05-20

文章信息/Info

Title:
Detection of endocannabinoids in saliva and hair by high performance liquid chromatography tandem mass spectrometry
作者:
陈盛火张权Mwanza Christopher吴彦段彩灵邓慧华
东南大学儿童发展与学习科学教育部重点实验室, 南京 210096; 东南大学学习科学研究中心, 南京 210096
Author(s):
Chen Shenghuo Zhang Quan Mwanza Christopher Wu Yan Duan Cailing Deng Huihua
Key Laboratory of Child Development and Learning Science of Ministry of Education, Southeast University, Nanjing 210096, China
Research Center of Learning Science, Southeast University, Nanjing 210096, China
关键词:
高效液相色谱-串联质谱 AEA 2-AG 唾液 头发
Keywords:
high performance liquid chromatography tandem mass spectrometry N-arachidonoyl-ethanolamine(AEA) 2-arachidonoylglycerol(2-AG) saliva hair
分类号:
O657.63
DOI:
10.3969/j.issn.1001-0505.2017.03.020
摘要:
基于高效液相色谱-串联质谱仪建立了检测唾液和头发中花生四烯酸乙醇氨(AEA)和2-花生四烯酸甘油(2-AG)含量的方法,并用于检测157名在校大学生唾液和头发中的AEA和2-AG,探讨AEA和2-AG对性别和肥胖的影响.唾液中的待测物采用乙酸乙酯液液萃取提纯;头发中的待测物经甲醇孵育,用固相萃取柱萃取提纯.在大气压化学电离正离子模式和多反应监测方式下进行定性测试,以外标法进行定量测试.方法验证结果显示,唾液AEA,2-AG检测限分别为0.07和0.40 ng/mL;头发AEA,2-AG检测限分别为0.7和3.2 pg/mg;日内、日间变异系数均小于15%,回收率为97.9%~108.0%.人群研究结果表明,肥胖者唾液和头发AEA,2-AG含量均显著高于非肥胖者(p<0.010);男性唾液2-AG含量显著低于女性(p<0.050),但AEA含量没有性别差异(p=0.844);男性头发2-AG含量显著高于女性(p<0.010),但AEA含量没有性别差异(p=0.456).
Abstract:
A new method based on high performance-liquid chromatography tandem mass spectrometry was developed for quantification of N-arachidonoyl-ethanolamine(AEA)and 2-arachidonoyl-glycerol(2-AG)in saliva and hair. This method was used for 157 healthy undergraduate students to explore the influences of gender and obesity on the contents of AEA and 2-AG. The analysts in saliva were extracted by liquid-liquid extraction with ethyl acetate and those in hair were purified by solid phase extraction with methanol. The qualitative tests were carried out with atmosphere pressure chemical ionization in the positive mode and multiple reaction monitoring, while the quantitative tests were performed with the external standard method. The method validation results reveal that the detection limits for saliva AEA and 2-AG are 0.07 and 0.40 ng/mL, respectively, and those for hair AEA and 2-AG are 0.7 and 3.2 pg/mg, respectively. The intra-day and inter-day coefficients of variation are less than 15% and the recovery ranges from 97.9% to 108.0%. The population analysis results show that the contents of AEA and 2-AG of salivary and hair of the obese individuals are significantly than those of the non-obese ones(p<0.010). The contents of saliva 2-AG of males are obviously lower than those of females(p<0.050), but no gender difference in AEA content is observed(p=0.844). The contents of hair 2-AG of males are obviously higher than those of females(p<0.01), but there is no significant gender difference in hair AEA(p=0.456).

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备注/Memo

备注/Memo:
收稿日期: 2016-10-26.
作者简介: 陈盛火(1990—),男,硕士生;邓慧华(联系人),男,博士,教授,博士生导师,dengrcls@seu.edu.cn.
基金项目: 教育部人文社会科学研究一般资助项目(15YJAZH009)、江苏省社会科学基金重点资助项目(15GLB017)、江苏省普通高校研究生科研创新计划资助项目(KYZZ15_0054)
引用本文: 陈盛火,张权,Mwanza Christopher,等.唾液及头发中内源性大麻素的高效液相色谱-串联质谱检测[J].东南大学学报(自然科学版),2017,47(3):534-538. DOI:10.3969/j.issn.1001-0505.2017.03.020.
更新日期/Last Update: 2017-05-20