[1]刘剀剡,丁勤,袁梦玹,等.鞘氨醇单胞菌对微囊藻毒素-RR的降解作用与影响因素分析[J].东南大学学报(自然科学版),2021,51(3):496-502.[doi:10.3969/j.issn.1001-0505.2021.03.019]
 Liu Kaiyan,Ding Qin,Yuan Mengxuan,et al.Biodegradation of Microcystin-RR by Sphingopyxis sp. and its influencing factors[J].Journal of Southeast University (Natural Science Edition),2021,51(3):496-502.[doi:10.3969/j.issn.1001-0505.2021.03.019]
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鞘氨醇单胞菌对微囊藻毒素-RR的降解作用与影响因素分析()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
51
期数:
2021年第3期
页码:
496-502
栏目:
环境科学与工程
出版日期:
2021-05-20

文章信息/Info

Title:
Biodegradation of Microcystin-RR by Sphingopyxis sp. and its influencing factors
作者:
刘剀剡丁勤袁梦玹浦跃朴
东南大学环境医学工程教育部重点实验室, 南京 210009
Author(s):
Liu Kaiyan Ding Qin Yuan Mengxuan Pu Yuepu
Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Southeast University, Nanjing 210009, China
关键词:
微囊藻毒素-RR 鞘氨醇单胞菌 生物降解 影响因素 机制
Keywords:
Microcystin-RR(MC-RR) Sphingopyxis sp. biodegradation influencing factors mechanisms
分类号:
X522
DOI:
10.3969/j.issn.1001-0505.2021.03.019
摘要:
为探索微囊藻毒素微生物降解机制,研究了鞘氨醇单胞菌对微囊藻毒素-RR(MC-RR)的降解作用与影响因素.通过色谱及质谱方法阐明了鞘氨醇单胞菌对MC-RR的降解能力及途径,结合实时荧光定量聚合酶链式反应技术和酶活性分析探究了反应温度、营养基质及同类毒素(微囊藻毒素-LR,MC-LR)的影响及机制.结果表明:鞘氨醇单胞菌对MC-RR的最高降解速率达0.29 mg/(L·h),降解产物分别为C49H77N13O13、C32H47N4O8、C12H19N3O6和C20H29NO3;温度影响酶MlrA活性,在20~40 ℃范围内最适温度为30 ℃;提高磷质量浓度(100 mg/L K2HPO4)可刺激mlrA基因表达,使降解速率增加27.59%;提高碳质量浓度(100 mg/L葡萄糖)将抑制mlrA表达,使降解速率下降86.71%;MC-LR和MC-RR竞争性结合MlrA,使MC-RR降解速率下降4.44%.鞘氨醇单胞菌对MC-RR的降解能力较强,并受到温度、营养基质及MC-LR浓度的影响.
Abstract:
To explore the microbial degradation mechanism of Microcystins, the degradation of Microcystin-RR(MC-RR)by Sphingopyxis sp. and its influencing factors were studied. The degradation ability and the pathway of MC-RR by Sphingopyxis sp. were clarified by chromatography and mass spectrometry. Combined with real-time fluorescent quantitative polymerase chain reaction technology and the analysis of enzyme activity, the effects and mechanisms of reaction temperature, nutrient matrix and similar toxins(Microcystin-LR, MC-LR)were investigated. The results show that the highest degradation rate of MC-RR is 0.29 mg/(L·h). The degradation products are C49H77N13O13, C32H47N4O8, C12H19N3O6, and C20H29NO3. The temperature affects the activity of MlrA enzyme and the optimum temperature is 30 ℃ at 20-40 ℃. Increasing phosphorus concentration(100 mg/L K2HPO4)can stimulate mlrA gene expression and increase the degradation rate by 27.59%. Increasing the carbon concentration(100 mg/L glucose)can inhibit mlrA gene expression and reduce the degradation rate by 86.71%. MC-LR and MC-RR are competitively combined with MlrA, the degradation rate of MC-RR is reduced by 4.44%. The Sphingopyxis sp. has strong ability to degrade MC-RR, thus it is affected by the temperature, the nutrient matrix and the MC-LR concentration.

参考文献/References:

[1] Imanishi S,Kato H,Mizuno M,et al.Bacterial degradation of microcystins and nodularin[J].Chemical Research in Toxicology,2005,18(3):591-598.DOI:10.1021/tx049677g.
[2] 贺燕,黄先智,丁晓雯.微囊藻毒素毒性及其作用机理研究进展[J].食品科学,2020,41(5):290-298.DOI:10.7506/spkx1002-6630-20190408-078.
He Y,Huang X Z,Ding X W.Advances in research on toxicity and mechanism of action of microcystins[J].Food Science,2020,41(5):290-298.DOI:10.7506/spkx1002-6630-20190408-078. (in Chinese)
[3] Diez-Quijada L,Puerto M,Gutierrez-Praena D,et al.Microcystin-RR:Occurrence,content in water and food and toxicological studies.A review[J].Environmental Research,2019,168:467-489.DOI:10.1016/j.envres.2018.07.019.
[4] Preece E P,Hardy F J,Moore B C,et al.A review of microcystin detections in Estuarine and Marine waters:Environmental implications and human health risk[J].Harmful Algae,2017,61:31-45.DOI:10.1016/j.hal.2016.11.006.
[5] 袁强,余丽,李卫华,等.巢湖和太湖春季微囊藻毒素动态差异及与环境因子的关系[J].环境工程技术学报,2021,11(2):267-277.DOI:10.12153/j.issn.1674-991X.20200225.
Yuan Q,Yu L,Li W H,et al.Dynamic differences of microcystins and their relationship with environmental factors in spring in Lake Chaohu and Lake Taihu[J].Journal of Environmental Engineering Technology,2021,11(2):267-277.DOI:10.12153/j.issn.1674-991X.20200225. (in Chinese)
[6] 舒秀波,谢丽强,万翔,等.太湖梅梁湾水体及沉积物中微囊藻毒素含量垂向分布特征[J].湖泊科学,2019,31(4):976-987.DOI:10.18307/2019.0426.
Shu X B,Xie L Q,Wan X,et al.Vertical distribution characteristics of microcystin concentration in water and sediment of Meiliang Bay,Lake Taihu[J].Journal of Lake Sciences,2019,31(4):976-987.DOI:10.18307/2019.0426. (in Chinese)
[7] 李祝,郭向前,赵以军,等.水环境中微囊藻毒素的生物降解[J].生态科学,2005,24(1):90-95.DOI:10.3969/j.issn.1008-8873.2005.01.024.
Li Z,Guo X Q,Zhao Y J,et al.Biodegradation of microcystin in aquatic environment[J].Ecologic Science,2005,24(1):90-95.DOI:10.3969/j.issn.1008-8873.2005.01.024. (in Chinese)
[8] 王昊,徐立红.微囊藻毒素研究的当前进展和未来方向[J].水生生物学报,2011,35(3):504-515.DOI:10.3724/SP.J.1035.2011.00504.
Wang H,Xu L H.The current developments and future directions in microcystins study[J].Acta Hydrobiologica Sinica,2011,35(3):504-515.DOI:10.3724/SP.J.1035.2011.00504. (in Chinese)
[9] Zhang J,Lu Q Q,Ding Q,et al.A novel and native microcystin-degrading bacterium of Sphingopyxis sp.isolated from Lake Taihu[J].International Journal of Environmental Research and Public Health,2017,14(10):1187.DOI:10.3390/ijerph14101187.
[10] Maghsoudi E,Fortin N,Greer C,et al.Cyanotoxin degradation activity and mlr gene expression profiles of a Sphingopyxis sp.isolated from Lake Champlain,Canada[J].Environmental Science:Processes & Impacts,2016,18(11):1417-1426.DOI:10.1039/c6em00001k.
[11] Bourne D G,Riddles P,Jones G J,et al.Characterisation of a gene cluster involved in bacterial degradation of the cyanobacterial toxin microcystin LR[J].Environmental Toxicology,2001,16(6):523-534.DOI:10.1002/tox.10013.
[12] 潘禹,王华生,詹鸿峰,等.微囊藻毒素降解酶MlrA的生物学特征及催化机理研究进展[J].化工学报,2020,71(3):945-954.DOI:10.11949/0438-1157.20191008.
Pan Y,Wang H S,Zhan H F,et al.Progress of biological characterization and mechanism of enzymatic degradation of microcystinase[J].CIESC Journal,2020,71(3):945-954.DOI:10.11949/0438-1157.20191008. (in Chinese)
[13] Wang R P,Li J M,Jiang Y G,et al.Heterologous expression of mlrA gene originated from Novosphingobium sp.THN1 to degrade microcystin-RR and identify the first step involved in degradation pathway[J].Chemosphere,2017,184:159-167.DOI:10.1016/j.chemosphere.2017.05.086.
[14] Ding Q,Liu K Y,Xu K,et al.Further understanding of degradation pathways of microcystin-LR by an indigenous Sphingopyxis sp.in environmentally relevant pollution concentrations[J].Toxins,2018,10(12):536.DOI:10.3390/toxins10120536.
[15] Li H,Ai H N,Kang L,et al.Simultaneous microcystis algicidal and microcystin degrading capability by a single acinetobacter bacterial strain[J].Environmental Science & Technology,2016,50(21):11903-11911.DOI:10.1021/acs.est.6b03986.
[16] Scherer P I,Raeder U,Geist J,et al.Influence of temperature,mixing,and addition of microcystin-LR on microcystin gene expression in Microcystis aeruginosa[J].MicrobiologyOpen,2017,6(1):e00393.DOI:10.1002/mbo3.393.
[17] 刘凯英,薛罡,程起跃,等.微囊藻毒素-RR高效降解菌的分离鉴定及降解特性[J].环境科学与技术,2012,35(4):22-26.
  Liu K Y,Xue G,Cheng Q Y,et al.Isolation and identification of a microcystin-RR degrading bacterium and its biodegradation characteristics[J].Environmental Science & Technology,2012,35(4):22-26.(in Chinese)
[18] Yang F,Zhou Y L,Sun R L,et al.Biodegradation of microcystin-LR and-RR by a novel microcystin-degrading bacterium isolated from Lake Taihu[J].Biodegradation,2014,25(3):447-457.DOI:10.1007/s10532-013-9673-y.
[19] Wan X,Steinman A D,Gu Y R,et al.Occurrence and risk assessment of microcystin and its relationship with environmental factors in lakes of the eastern plain ecoregion,China[J].Environmental Science and Pollution Research,2020,27(36):45095-45107.DOI:10.1007/s11356-020-10384-0.
[20] Zhu X Y,Shen Y T,Chen X G,et al.Biodegradation mechanism of microcystin-LR by a novel isolate of Rhizobium sp.TH and the evolutionary origin of the mlrA gene[J].International Biodeterioration & Biodegradation,2016,115:17-25.DOI:10.1016/j.ibiod.2016.07.011.
[21] Li W,He J,Chen J,et al.Excretion pattern and dynamics of glutathione detoxification of microcystins in Sprague Dawley rat[J].Chemosphere,2018,191:357-364.DOI:10.1016/j.chemosphere.2017.09.083.
[22] 张运林,秦伯强,朱广伟.过去40年太湖剧烈的湖泊物理环境变化及其潜在生态环境意义[J].湖泊科学,2020,32(5):1348-1359.DOI:10.18307/2020.0503.
Zhang Y L,Qin B Q,Zhu G W.Long-term changes in physical environments and potential implications for the eco-environment of Lake Taihu in the past four decades[J].Journal of Lake Sciences,2020,32(5):1348-1359.DOI:10.18307/2020.0503. (in Chinese)
[23] 蔡启铭.太湖环境生态研究(一)[M].北京:气象出版社,1998:77-79.
[24] Nagar V,Pansare Godambe L,Bandekar J R,et al.Biofilm formation by Aeromonas strains under food-related environmental stress conditions[J].Journal of Food Processing and Preservation,2017,41(5):e13182.DOI:10.1111/jfpp.13182.
[25] Zilliges Y,Kehr J C,Mikkat S,et al.An extracellular glycoprotein is implicated in cell-cell contacts in the toxic cyanobacterium Microcystis aeruginosa PCC 7806[J].Journal of Bacteriology,2008,190(8):2871-2879.DOI:10.1128/jb.01867-07.
[26] Shimizu K,Maseda H,Okano K,et al.How microcystin-degrading bacteria express microcystin degradation activity[J].Lakes & Reservoirs:Research & Management,2011,16(3):169-178.DOI:10.1111/j.1440-1770.2011.00480.x.
[27] Jiang Y G,Shao J H,Wu X Q,et al.Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu,China[J].FEMS Microbiology Letters,2011,322(2):108-114.DOI:10.1111/j.1574-6968.2011.02337.x.
[28] 潘禹,王华生,詹鸿峰,等.微囊藻毒素降解酶MlrA的结构功能分析[J].化工学报,2021,72(3):1643-1653.DOI:10.11949/0438-1157.20200729.
Pan Y,Wang H S,Zhan H F,et al.Structural and functional analysis of MlrA from microcystin-degrading bacteria[J].CIESC Journal,2021,72(3):1643-1653.DOI:10.11949/0438-1157.20200729. (in Chinese)
[29] Chen J,Hu L B,Zhou W,et al.Degradation of microcystin-LR and RR by a Stenotrophomonas sp.strain EMS isolated from Lake Taihu,China[J].International Journal of Molecular Sciences,2010,11(3):896-911.DOI:10.3390/ijms11030896.

备注/Memo

备注/Memo:
收稿日期: 2021-01-07.
作者简介: 刘剀剡(1994—),女,硕士生;浦跃朴(联系人),男,博士,教授,博士生导师,yppu@seu.edu.cn.
基金项目: 国家自然科学基金面上资助项目(81972997).
引用本文: 刘剀剡,丁勤,袁梦玹,等.鞘氨醇单胞菌对微囊藻毒素-RR的降解作用与影响因素分析[J].东南大学学报(自然科学版),2021,51(3):496-502. DOI:10.3969/j.issn.1001-0505.2021.03.019.
更新日期/Last Update: 2021-05-20