[1]吕晓迎,黄蕙芬,唐祖明,等.光反射干涉生物传感器对生物医用材料蛋白质吸附研究[J].东南大学学报(自然科学版),2001,31(2):13-17.[doi:10.3969/j.issn.1001-0505.2001.02.004]
　Lü Xiaoying,Huang Huifen,Tang Zuming,et al.Reflectometry Interference Spectroscopy for Study of Protein Adsorption on Biomaterials[J].Journal of Southeast University (Natural Science Edition),2001,31(2):13-17.[doi:10.3969/j.issn.1001-0505.2001.02.004]
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光反射干涉生物传感器对生物医用材料蛋白质吸附研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
31
期数:
2001年第2期
页码:
13-17
栏目:
生物医学工程
出版日期:
2001-03-20

文章信息/Info

Title:
Reflectometry Interference Spectroscopy for Study of Protein Adsorption on Biomaterials
作者:
吕晓迎1;  黄蕙芬2;  唐祖明1;  沈晓峰1;  张浩康2;  陈德敏3;  钟银屏4;  俞枋1;  王德育1;  黄炎1;  钱卫平1;  袁春伟1
1 东南大学生物科学与医学工程系, 南京　210096; 2 东南大学电子工程系, 南京　210096; 3 上海生物材料研究测试中心, 上海　200023; 4 四川大学高分子材料系, 成都　610065
Author(s):
Lü Xiaoying1;  Huang Huifen2;  Tang Zuming1;  Sheng Xiaofeng1;  Zhang Haokang1;  Chen Demin3 Zhong Yinping4;  Yu Fang1;  Wang Deyu1;  Huang Yan1;  Qian Weiping1;  Yuan Chunwei1
1 Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096; 2 Department of Electronic Engineering, Southeast University, Nanjing 210096; 3 Shanghai Study and Test Center of Biomaterials, Shanghai 200023; 4 Department of Macromolecule Materials, Si-Chuan University, Chengdu 610065)
关键词:
光反射干涉生物传感器;  羟基磷灰石;  聚氨酯;  蛋白吸附;  生物相容性
Keywords:
RIfS;  hydroxyapatite;  polyurethane;  protein adsorption;  biocompatibility
分类号:
R318.08
DOI:
10.3969/j.issn.1001-0505.2001.02.004
摘要:
应用东南大学生物医学工程系吴健雄实验室研制的RIfS生物传感器,对人工合成羟基磷灰石和H50-50型聚氨酯两种材料对小牛血清白蛋白BSA、人血浆纤维蛋白原FIB和人免疫球蛋白IgG的吸附性能进行了实时原位动态研究.根据RIfS表征原理和蛋白质结构大小,引入了吸附层数计算公式,求出了2种实验材料对不同蛋白质的吸附层数.得出羟基磷灰石和H50-50型聚氨酯材料对3种蛋白质实际吸附层数分别为:IgG 1.075 1, BSA 0.968 4,FIB 0.746 4和IgG 0.819 9, BSA 0.796 4,FIB 0.612 0.证实了用RIfS生物传感器进行生物材料生物相容性研究的可行性.
Abstract:
Using a RIfS set-up made by our laboratory we have investigated the kinetic behavior of bovine serum albumin (BSA), human fibrinogen (FIB) and human IgG adsorbed onto surfaces of hydroxyapatite and polyurethane H50-50. According to the principle of operation of RIfS and the molecular dimensions of three kinds of proteins we introduced a formula to calculate the adsorbed layers of proteins onto hydroxyapatite and polyurethane H50-50. The results show that the adsorbed layers of three kinds of proteins on the surface of hydroxyapatite are: IgG 1.075 1, BSA 0.968 4,FIB 0.746 4, and of polyurethane H50-50 are: IgG 0.819 9, BSA 0.796 4,FIB 0.612 0. The potential application of RIfS as a new analytical tool in the evaluation of the biocompatibility of biomaterials was confirmed.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:吕晓迎,女,1956年生,副教授.
基金项目:国家教委留学回国人员科研资助项目.
顾汉卿.生物材料、人工器官与组织工程.第六届全国生物材料学术研讨会.广东番禺.1999.
更新日期/Last Update: 2001-03-20