[1]周士新,谢建明,孙啸,等.不同物种间胰岛素及其编码mRNA,DNA序列比较与分析[J].东南大学学报(自然科学版),2003,33(3):292-295.[doi:10.3969/j.issn.1001-0505.2003.03.011]
　Zhou Shixin,Xie Jianming,Sun Xiao,et al.Comparison and analysis of insulin sequences and their coded mRNA and DNA among different species[J].Journal of Southeast University (Natural Science Edition),2003,33(3):292-295.[doi:10.3969/j.issn.1001-0505.2003.03.011]
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不同物种间胰岛素及其编码mRNA,DNA序列比较与分析()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
33
期数:
2003年第3期
页码:
292-295
栏目:
生物医学工程
出版日期:
2003-05-20

文章信息/Info

Title:
Comparison and analysis of insulin sequences and their coded mRNA and DNA among different species
作者:
周士新;  谢建明;  孙啸;  陆祖宏
东南大学分子与生物分子电子学教育部重点实验室,南京 210096
Author(s):
Zhou Shixin;  Xie Jianming;  Sun Xiao;  Lu zuhong
Key Laboratory of Molecular and Bio-molecular Electronics of Ministry of Education, Southeast University, Nanjing 210096, China
关键词:
胰岛素;  蛋白质(氨基酸)序列;  信使RNA;  DNA;  C肽
Keywords:
insulin;  protein(amino acids)sequences;  mRNA;  DNA;  C-peptide
分类号:
Q617
DOI:
10.3969/j.issn.1001-0505.2003.03.011
摘要:
通过PSI-BLAST搜索与人类胰岛素原(含有86个氨基酸)相似的蛋白质序列,并进行比对,计算比对矩阵的相似得分和期望值,同时运用ClustalW算法对不同物种编码前胰岛素原mRNA及其翻译的蛋白质和DNA序列进行多重比对.结果发现,脊椎动物的胰岛素蛋白质一级结构(A链和B链)和mRNA非常相似,但部分动物C肽的部分序列有差异; 系统进化分析表明,人和猴、小鼠和大鼠编码胰岛素的mRNA在进化上关系相近.各物种间编码相同氨基酸的核苷酸序列(CDS)相同,但编码胰岛素的DNA序列不同.各物种胰岛素原蛋白质序列中,A链和B链序列保守,C肽有一定的差异; DNA序列差异较大.
Abstract:
In order to understand the sequences of insulin(protein), mRNA and DNA among different species, PSI-BLAST was used to search for the similar protein sequences on the basis of human proinsulin(86 amino acids), and the sequences alignments were made. Meanwhile, the ClustalW program was utilized to make multiple sequences alignment of coding mRNA, translated preproinsulin, and DNA of insulin. The results indicate that the insulin(A chain and B chain)primary protein sequences and mRNA of vertebrate are quite similar. But C-peptide of some animals show some differences in part of amino acid sequences. Analysis of phylogenetic tree indicates that human and monkey, mouse and rat are quite similar in the evolution. There are obvious differences between human insulin DNA sequences and those of mouse or rat. However, the CDS mRNA of varied species coding the same amino acids are homology. The conclusion is that insulin(A, B chains)sequences are conservative, however the sequences of C-peptide have some difference among human and animals, while the DNA sequences have obvious difference in human, mouse and rat.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金“创新科学基金”资助项目(60121101)、国家高科技发展计划(863计划)资助项目(2002AA231071).
作者简介: 周士新(1968—),男,博士生,主管医师,qizhou98@seu.edu.cn; 陆祖宏(联系人),男,博士,教授,博士生导师,zhlu@seu.edu.cn.
更新日期/Last Update: 2003-05-20