[1]赵艳秋,浦跃朴,王军,等.牛血清白蛋白改性壳聚糖纤维及其细胞亲和性研究[J].东南大学学报(自然科学版),2003,33(4):479-482.[doi:10.3969/j.issn.1001-0505.2003.04.024]
 Zhao Yanqiu,Pu Yuepu,Wang Jun,et al.Study on chitosan fibers modified with bovine serum albumin and its cellular affinity[J].Journal of Southeast University (Natural Science Edition),2003,33(4):479-482.[doi:10.3969/j.issn.1001-0505.2003.04.024]
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牛血清白蛋白改性壳聚糖纤维及其细胞亲和性研究()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
33
期数:
2003年第4期
页码:
479-482
栏目:
生物医学工程
出版日期:
2003-07-20

文章信息/Info

Title:
Study on chitosan fibers modified with bovine serum albumin and its cellular affinity
作者:
赵艳秋1 浦跃朴1 王军2 李新松2 尹立红1
1 东南大学公共卫生学院, 南京 210009; 2 东南大学化学化工系, 南京 210096
Author(s):
Zhao Yanqiu1 Pu Yuepu1 Wang Jun2 Li Xinsong2 Yin Lihong1
1 School of Public Health, Southeast University, Nanjing 210009, China
2 Department of Chemistry and Chemical Engineering, Southeast University, Nanjing 210096, China
关键词:
壳聚糖 牛血清白蛋白 改性 细胞亲和性
Keywords:
chitosan bovine serum albumin modification cellular affinity
分类号:
Q81
DOI:
10.3969/j.issn.1001-0505.2003.04.024
摘要:
采用牛血清白蛋白对壳聚糖纤维进行化学改性,通过扫描电镜和含水量测定对改性的牛血清白蛋白-壳聚糖纤维进行表征.并对改性材料进行细胞相容性和细胞亲和性测定与评价.结果表明:牛血清白蛋白-壳聚糖纤维表面有明显附着物,纤维略微变粗; 含水量增加到93.49%(质量分数); 细胞在改性壳聚糖纤维上的形态无异常,粘附、生长、增殖情况良好; 细胞毒性试验结果呈阴性.该改性壳聚糖纤维具有良好的生物学特性,能够应用于器官组织工程及生物医药领域.
Abstract:
To improve cell adhesion and growth on chitosan fibers(CF), bovine serum albumin(BSA)was chemically grafted to its surface. The surface of CF was treated by periodate oxidation and then connected with BSA. Scan electron microscopy and water content measurement were performed to characterize the novel material(bovine serum albumin-chitosan fibers, BSA-CF). To evaluate the biocompatibility and cellular affinity of BSA-CF, cell toxicity, cell attachment and proliferation on BSA-CF were detected. The results show that some attachment on the surface of BSA-CF can be observed under scan electron spectroscopy. Water content is 93.49% in mass fraction and cell morphology and quantity on the matrix have no abnormity. The BSA-CF is proved to be with excellent biocompatibility and cellular affinity. In conclusion, the novel material is useful for the maintenance of cells on chemically defined dressing for enhancing tissue repair or matrix for tissue engineering.

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备注/Memo

备注/Memo:
基金项目: 教育科学技术研究重点资助项目(02113)、江苏省333工程基金资助项目(01012).
作者简介: 赵艳秋(1977—),女,硕士生; 浦跃朴(联系人),男,教授,博士生导师,yppu@seu.edu.cn.
更新日期/Last Update: 2003-07-20