[1]许燕波,钱春香,陆兆文.甘油提高巴氏芽孢杆菌脲酶的热稳定性[J].东南大学学报(自然科学版),2013,43(1):147-151.[doi:10.3969/j.issn.1001-0505.2013.01.028]
　Xu Yanbo,Qian Chunxiang,Lu Zhaowen.Study on enhancing urease thermostability of Bacillus pasteurii by glycerol[J].Journal of Southeast University (Natural Science Edition),2013,43(1):147-151.[doi:10.3969/j.issn.1001-0505.2013.01.028]
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甘油提高巴氏芽孢杆菌脲酶的热稳定性()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
43
期数:
2013年第1期
页码:
147-151
栏目:
生物医学工程
出版日期:
2013-01-20

文章信息/Info

Title:
Study on enhancing urease thermostability of Bacillus pasteurii by glycerol
作者:
许燕波; 钱春香; 陆兆文
东南大学材料科学与工程学院, 南京211189; 东南大学江苏省土木工程材料重点实验室, 南京211189
Author(s):
Xu Yanbo;  Qian Chunxiang;  Lu Zhaowen
School of Materials Science and Engineering Southeast University, Nanjing 211189, China
Jiangsu Key Laboratory of Construction Materials, Southeast University, Nanjing 211189, China
关键词:
碳酸盐矿化菌;  脲酶;  甘油;  热稳定性
Keywords:
carbonate-mineralization microbe;  urease;  glycerol;  thermostability
分类号:
Q89
DOI:
10.3969/j.issn.1001-0505.2013.01.028
摘要:
研究了保存时间和保存温度对碳酸盐矿化菌脲酶活性的影响,发现在低温4 ℃条件下,脲酶活性在29 d内出现不同程度下降;常温短期储存时,酶活性可以保持3 d,3 d后酶活性值下降明显.为提高脲酶的热稳定性,向碳酸盐矿化菌中添加甘油作为保护剂,常温下添加总体积分数20%的甘油,可以提高酶的稳定性,脲酶活性在30 ℃下3 d内保持不变,但甘油保护作用有限,高温储存超过7 d后酶活性值下降明显;而在保存温度4 ℃条件下,添加体积分数为10%的甘油,碳酸盐矿化菌可以储存30 d,底物分解量可以达到初始菌液的95%.
Abstract:
Effect of preservation time and temperature on the carbonate-mineralization microbe urease activity is studied. The urease activity decreases in 29 days at 4 ℃ while it can be kept for 3 days at normal temperature before obvious decrease afterwards. Glycerol is selected as the protective additive to enhance the urease thermostability. The urease activity keep stable for 3 days at 30 ℃, and the best volume fraction for glycerol is 20% at normal temperature. However its effect of preservation is limited, and the urease activity decreases obviously after 7 days at high store temperature. The decomposition ability can reach up to 95% at 4 ℃ after 30 days with 10% glycerol, and the carbonate-mineralization microbe can be kept in reserve for a month.

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备注/Memo

备注/Memo:
作者简介: 许燕波(1986—),男,硕士生;钱春香(联系人),女,博士,教授,博士生导师, cxqian@seu.edu.cn.
基金项目: 江苏省科技支撑计划资助项目(BK2010062).
引文格式: 许燕波,钱春香,陆兆文.甘油提高巴氏芽孢杆菌脲酶的热稳定性［J］.东南大学学报:自然科学版,2013,43(1):147-151. ［doi:10.3969/j.issn.1001-0505.2013.01.028］
更新日期/Last Update: 2013-01-20