[1]杨小丽,周娜,陈明,等.FISH技术解析不同氨氮浓度MBR中的微生物群落结构[J].东南大学学报(自然科学版),2013,43(2):380-385.[doi:10.3969/j.issn.1001-0505.2013.02.029]
　Yang Xiaoli,Zhou Na,Chen Ming,et al.Analysis of microbial community structure in MBR with different ammonia concentrations using fluorescence in situ hybridization[J].Journal of Southeast University (Natural Science Edition),2013,43(2):380-385.[doi:10.3969/j.issn.1001-0505.2013.02.029]
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FISH技术解析不同氨氮浓度MBR中的微生物群落结构()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
43
期数:
2013年第2期
页码:
380-385
栏目:
环境科学与工程
出版日期:
2013-03-20

文章信息/Info

Title:
Analysis of microbial community structure in MBR with different ammonia concentrations using fluorescence in situ hybridization
作者:
杨小丽1; 周娜1; 陈明1; 张瑞1; 宋海亮2; 傅大放1
1东南大学土木工程学院, 南京 210096; 2东南大学能源与环境学院, 南京 210096
Author(s):
Yang Xiaoli1;  Zhou Na1;  Chen Ming1;  Zhang Rui1;  Song Hailiang2;  Fu Dafang1
1School of Civil Engineering, Southeast University, Nanjing 210096, China
2School of Energy and Environment, Southeast University, Nanjing 210096, China
关键词:
膜生物反应器;  荧光原位杂交;  微生物群落结构;  变形菌;  氨氧化细菌;  硝化细菌
Keywords:
membrane bioreactor;  fluorescence in situ hybridization;  microbial community structure;  Proteobacteria;  Ammonia-oxidizing bacteria;  Nitrobacter sp.
分类号:
X703.1
DOI:
10.3969/j.issn.1001-0505.2013.02.029
摘要:
为了探究膜生物反应器(MBR)中微生物群落结构与硝化作用的内在关系,采用荧光原位杂交技术(FISH)对不同氨氮浓度的MBR系统中微生物种群进行检测,考察变形菌(Proteobacteria)、放线菌(Actinobacteria)、黄杆菌(Cytophaga-flexibacter-bacteroides)、绿弯菌(Chloroflexi)、硝化细菌(Nitrobacter sp.)和氨氧化细菌(Ammonia-oxidizing bacteria)占总细菌的相对含量.结果表明:进水氨氮浓度分别为35,3.47和100 mg/L的MBR系统中,变形菌分别占总细菌的51%,44%和39%,氨氧化细菌与硝化细菌之和分别占总细菌的28%,4%和43%.在氨氮浓度较低的MBR中,检测到大量丝状β-变形菌,同时放线菌和绿弯菌也有所增加.高氨氮负荷有利于氨氧化细菌和硝化细菌的生长富集,并使其在系统中成为优势种群.相关性分析表明,氨氧化细菌、硝化细菌与氨氮去除呈显著正相关.
Abstract:
The intrinsic relationship between the microbial community structure and nitrification was investigated in the membrane bioreactor(MBR)with different influent NH+4-N concentrations. Fluorescence in situ hybridization(FISH)was adopted for analyzing microbial community structure. Proteobacteria, Actinobacteria, Cytophaga-flexibacter-bacteroides, Chloroflexi, Nitrobacter sp. and Ammonia-oxidizing bacteria were detected. The results show that when the influent NH+4-N concentrations are 35, 3.47 and 100 mg/L, Proteobacteria accounts for 51%, 44% and 39% of total bacteria, while the sum of Ammonia-oxidizing and Nitrobacter sp. accounts for 28%, 4% and 43%, respectively. Abundant filamentous Betaproteobacteria is detected in MBR with lower NH+4-N concentration. Actinobacteria and Chloroflexi are also found to be increased simultaneously. Ammonia-oxidizing bacteria and Nitrobacter sp. are enriched and dominant in MBR with higher NH+4-N load. Correlation analysis indicates that Ammonia-oxidizing bacteria and Nitrobacter sp. are positively correlated with NH+4-N removal.

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备注/Memo

备注/Memo:
作者简介: 杨小丽(1977—),女,博士,副教授,yangxiaoli@seu.edu.cn.
基金项目: 国家自然科学基金资助项目(51008064,51109038)、江苏省高校优势学科建设工程资助项目(1105007001)、东南大学重大科学研究引导基金资助项目(3205002107).
引文格式: 杨小丽,周娜,陈明,等.FISH技术解析不同氨氮浓度MBR中的微生物群落结构［J］.东南大学学报:自然科学版,2013,43(2):380-385. ［doi:10.3969/j.issn.1001-0505.2013.02.029］
更新日期/Last Update: 2013-03-20