[1]夏阳,陈慧敏,胡姝颖,等.纳米αFe2O3对牙髓干细胞增殖及骨向分化和矿化能力的影响[J].东南大学学报(自然科学版),2018,48(1):165-169.[doi:10.3969/j.issn.1001-0505.2018.01.025]
 Xia Yang,Chen Huimin,Hu Shuying,et al.Effects of nano αFe2O3 on proliferation, osteogenic differentiation and mineral synthesis of dental pulp stem cells[J].Journal of Southeast University (Natural Science Edition),2018,48(1):165-169.[doi:10.3969/j.issn.1001-0505.2018.01.025]
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纳米αFe2O3对牙髓干细胞增殖及骨向分化和矿化能力的影响()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
48
期数:
2018年第1期
页码:
165-169
栏目:
生物医学工程
出版日期:
2018-01-20

文章信息/Info

Title:
Effects of nano αFe2O3 on proliferation, osteogenic differentiation and mineral synthesis of dental pulp stem cells
作者:
夏阳12陈慧敏2胡姝颖2孙剑飞1章非敏23顾宁13
1东南大学江苏省生物材料与器件重点实验室, 南京 210009; 2南京医科大学口腔疾病研究江苏省重点实验室, 南京 210029; 3苏州纳米科技协同创新中心, 苏州 215123
Author(s):
Xia Yang12 Chen Huimin2 Hu Shuying2 Sun Jianfei1 Zhang Feimin23 Gu Ning13
1Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing 210096, China
2Jiangsu Key Laboratory of Oral Disease, Nanjing Medical University, Nanjing 210029, China
3Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou 215123, China
关键词:
纳米αFe2O3 牙髓干细胞 增殖 骨向分化
Keywords:
nano αFe2O3 dental pulp stem cells proliferation osteogenic differentiation
分类号:
R780.2
DOI:
10.3969/j.issn.1001-0505.2018.01.025
摘要:
对纳米αFe2O3进行二巯基丁二酸表面修饰,采用透射电子显微镜(TEM)观察其形貌,利用振动样品磁强计(VSM)检测其磁学性能.配置含纳米αFe2O3浓度为10 μg/mL的培养基培养牙髓干细胞,以不添加纳米αFe2O3的培养基为对照组.采用荧光显微镜观察纳米αFe2O3组与常规培养基组的细胞形态,以CCK-8检测细胞增殖情况,碱性磷酸酶(ALP)试剂盒检测细胞ALP活性,并进行茜素红染色以检验其矿化能力.结果表明,所用纳米αFe2O3为棒状,尺寸约10 nm×90 nm,VSM结果显示其没有磁性.纳米αFe2O3培养基中牙髓干细胞的形态和增殖情况与常规培养基中情况没有差异,但是碱性磷酸酶活性和矿化能力则显著提高(p<0.05).由此说明,纳米αFe2O3对牙髓干细胞的形态和增殖没有影响,但却会促进其向成骨方向分化.
Abstract:
Nano αFe2O3 is surface modified with dimercaptosuccinic acid(DMSA). Its morphology is observed by transmission electronic microscopy(TEM), and its magnetic property is detected by vibration sample magnetometer(VSM). The media with 10 μg/mL nano αFe2O3 is used to culture dental pulp stem cells(DPSCs)by using the media without nano αFe2O3 as the control. The morphology of DPSCs in the nano αFe2O3 group and the control group is detected by fluorescent stain. The cell proliferation is detected by CCK-8, and the cell alkaline phosphatase activity(ALP)is measured by a ALP kit. Alizarin red staining(ARS)is used to detect the mineral synthesis. The results show that nano αFe2O3 is spindle with about 10 nm×90 nm in dimension. The VSM results prove that nano αFe2O3 is nonmagnetic. No significant difference in cell morphology and proliferation between the nano αFe2O3 group and the normal media group is detected. However, both ALP activity and mineralization formation of cells are significantly increased(p<0.05). Therefore, nano αFe2O3 does not affect the cell morphology and proliferation, but promotes the osteogenic differentiation of DPSCs.

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备注/Memo

备注/Memo:
收稿日期: 2017-07-04.
作者简介: 夏阳(1981—),女,博士,副教授;顾宁(联系人),男,博士,教授,博士生导师,guning@seu.edu.cn.
基金项目: 国家自然科学基金资助项目(81771044)、中国博士后基金资助项目(2015M571647)、江苏省博士后基金资助项目(1402044B)、江苏高校优势学科建设工程资助项目(2014-37)、江苏省青年医学人才资助项目(QNRC2016853).
引用本文: 夏阳,陈慧敏,胡姝颖,等.纳米αFe2O3对牙髓干细胞增殖及骨向分化和矿化能力的影响[J].东南大学学报(自然科学版),2018,48(1):165-169. DOI:10.3969/j.issn.1001-0505.2018.01.025.
更新日期/Last Update: 2018-01-20