[1]唐光媛,王洁,尹洁晨,等.饮用水遗传毒性评价方法建立和应用[J].东南大学学报(自然科学版),2018,48(1):170-174.[doi:10.3969/j.issn.1001-0505.2018.01.026]
 Tang Guangyuan,Wang Jie,Yin Jiechen,et al.Establishment and application of evaluation method for genotoxicity of drinking water[J].Journal of Southeast University (Natural Science Edition),2018,48(1):170-174.[doi:10.3969/j.issn.1001-0505.2018.01.026]
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饮用水遗传毒性评价方法建立和应用()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
48
期数:
2018年第1期
页码:
170-174
栏目:
环境科学与工程
出版日期:
2018-01-20

文章信息/Info

Title:
Establishment and application of evaluation method for genotoxicity of drinking water
作者:
唐光媛1王洁2尹洁晨1严庆1龚煜范1焦志刚1曹猛1黄书奇1朱光灿2刘冉1
1东南大学公共卫生学院, 南京 210009; 2东南大学能源与环境学院, 南京 210096
Author(s):
Tang Guangyuan1 Wang Jie2 Yin Jiechen1 Yan Qing1 Gong Yufan1Jiao Zhigang1 Cao Meng1 Huang Shuqi1 Zhu Guangcan2 Liu Ran1
1School of Public Health, Southeast University, Nanjing 210009, China
2School of Energy and Environment, Southeast University, Nanjing 210096, China
关键词:
Ames试验 微核试验 彗星试验 饮用水 遗传毒性
Keywords:
Ames test micronucleus test comet assay drinking water genotoxicity
分类号:
X172
DOI:
10.3969/j.issn.1001-0505.2018.01.026
摘要:
建立了一组多效应终点、多层次的饮用水遗传毒性测试体系, 并用于盐城某水厂饮用水处理工艺的评价. 采用Ames试验菌株TA98和TA100检测水样浓集物致突变效应, 分别利用人外周血淋巴细胞微核试验和彗星试验检测染色体结构损伤和DNA链断裂程度. Ames试验结果显示,水样浓集物在0.5~2.0 L/皿的浓度范围内未出现致突变性.微核试验中原水、砂滤水和出厂水均表现出较强的致染色体畸变作用, 0.125 L的剂量即可引起淋巴细胞微核细胞率的升高, 经过臭氧-生物活性炭处理后各剂量均与对照无差异. 彗星试验结果显示, 各水样均在不同剂量下对淋巴细胞DNA造成损伤, 原水、出厂水、砂滤水、生物活性炭(BAC)水的作用剂量分别为0.25,0.25,0.50和1.00 L, 损伤程度从大到小依次为原水、出厂水、砂滤水、BAC水.
Abstract:
A set of multi-effect endpoints and multi-level drinking water genotoxicity test system was established and applied to evaluate the drinking water treatment process in a water plant in Yancheng. Ames tester strains TA98 and TA100 were used to detect the mutagenic effects of water extracts. The micronucleus test and the comet assay of human peripheral blood lymphocyte were applied to detect chromosome breakage and DNA damage. The results of the Ames test show that mutagenicity is not observed in the range of 0.5 to 2.0 L/plate of water samples. The results of the micronucleus test exhibit that raw water, sand filter water and treated water are strong clastogenic, and can increase the lymphocyte micronucleus cell rate with the dose of 0.125 L. After the treatment with ozone and biological activated carbon, each dose has no difference with the control. The results of the comet assay show that water samples can damage DNA of lymphocyte at different doses. The effecting doses of raw water, tap water, sand filter water, and biological activated carbon(BAC)water are 0.25, 0.25, 0.50,and 1.00 L. The degree of damage from large to small is raw water, tap water, sand filter water,and BAC water.

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备注/Memo

备注/Memo:
收稿日期: 2017-07-10.
作者简介: 唐光媛(1990—),女,硕士生;刘冉(联系人),女,博士,教授,博士生导师,ranliu@seu.edu.cn.
基金项目: 国家水体污染控制与治理科技重大专项资助项目(2014ZX07405002)、东南大学大学生科研训练计划资助项目(201710286125).
引用本文: 唐光媛,王洁,尹洁晨,等.饮用水遗传毒性评价方法建立和应用[J].东南大学学报(自然科学版),2018,48(1):170-174. DOI:10.3969/j.issn.1001-0505.2018.01.026.
更新日期/Last Update: 2018-01-20