[1]杨佩,许斌,孙清江.生物功能化碳量子点制备[J].东南大学学报(自然科学版),2018,48(4):766-771.[doi:10.3969/j.issn.1001-0505.2018.04.025]
 Yang Pei,Xu Bin,Sun Qingjiang.Synthesis of biofunctional carbon quantum dots[J].Journal of Southeast University (Natural Science Edition),2018,48(4):766-771.[doi:10.3969/j.issn.1001-0505.2018.04.025]
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生物功能化碳量子点制备()
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《东南大学学报(自然科学版)》[ISSN:1001-0505/CN:32-1178/N]

卷:
48
期数:
2018年第4期
页码:
766-771
栏目:
生物医学工程
出版日期:
2018-07-20

文章信息/Info

Title:
Synthesis of biofunctional carbon quantum dots
作者:
杨佩许斌孙清江
东南大学生物科学与医学工程学院, 南京210096; 东南大学生物电子学国家重点实验室, 南京210096
Author(s):
Yang Pei Xu Bin Sun Qingjiang
School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210096, China
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
关键词:
碳量子点 细胞荧光成像 生物功能化 细胞核靶向
Keywords:
carbon dots cellular fluorescenct imaging biocompatibility nuclear targeting
分类号:
R318.08
DOI:
10.3969/j.issn.1001-0505.2018.04.025
摘要:
为了实现碳量子点在细胞荧光成像方面的应用,采用共价修饰的方法对碳量子点进行生物功能化.采用水热法,以柠檬酸为碳源,乙二胺为钝化剂合成了蓝色荧光碳量子点.为了进一步实现碳量子点的共价偶联,对碳量子点进行羧基化处理,然后通过两步功能分子修饰完成生物功能化碳量子点的制备.采用透射电子显微镜、荧光和紫外分光光度计、红外光谱仪、电位粒度分析仪及荧光共聚焦显微镜研究了生物功能化碳量子点的性质和功能.实验结果表明:聚乙二醇(PEG)和核定位肽TAT通过酰胺化反应成功修饰至碳量子点上,叶酸(FA)通过酯化反应成功修饰至PEG末端,两步共价修饰完成了生物功能化碳量子点的制备.该生物功能化碳量子点具有电中性、小尺寸、低毒性和细胞核靶向的功能,适用于细胞荧光成像分析.
Abstract:
Carbon quantum dots are biofunctionalized using a covalent modification method to achieve the application of carbon quantum dots in cellular fluorescent imaging. Blue fluorescent carbon quantum dots were synthesized through the hydrothermal method using citric acid as carbon source and ethylenediamine as passivation agent. Carbon quantum dots were carbonxylized to achieve covalent coupling, followed by two-step functional molecular modification to complete the preparation of biofunctionalized carbon quantum dots. The properties and biofunctions were investigated by transmission electron microscope, fluorescence and ultraviolet spectrophotometer, infrared spectrometer, potential particle size analyzer and fluorescence confocal microscope. The experimental results show that poly(ethylene glycol)(PEG)and nuclear targeting peptide TAT(trans-activator trans-cription)are successfully modified on the carbon quantum dots via one-step amidation coupling chemistry, and folic acid(FA)is successfully modified at the end of PEG. The biofunctionalization of carbon quantum dots is prepared by two-step covalent modification. The biofunctional carbon quantum dots with neutral electrical property, small size, low toxicity, and nuclear targeting can be applied to cellular fluorescenct imaging.

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备注/Memo

备注/Memo:
收稿日期: 2018-01-30.
作者简介: 杨佩(1992—),男,硕士生;孙清江(联系人),男,博士,教授,博士生导师,sunqj@seu.edu.cn.
基金项目: 国家自然科学基金资助项目(21375015, 21775021).
引用本文: 杨佩,许斌,孙清江.生物功能化碳量子点制备[J].东南大学学报(自然科学版),2018,48(4):766-771. DOI:10.3969/j.issn.1001-0505.2018.04.025.
更新日期/Last Update: 2018-07-20